論文

2021年7月

Noninvasive, label-free, and quantitative monitoring of lipase kinetics using terahertz emission technology

BIOTECHNOLOGY AND BIOENGINEERING
  • Sharifun Nahar
  • ,
  • Ahmed Mohamed
  • ,
  • Xavier Ropagnol
  • ,
  • Amir Hassanpour
  • ,
  • Toshihiko Kiwa
  • ,
  • Tsuneyuki Ozaki
  • ,
  • Marc A. Gauthier

118
11
開始ページ
4246
終了ページ
4254
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/bit.27893
出版者・発行元
WILEY

Enzymes catalyze chemical transformations of great importance in many fields, and analysis of the rate of these transformations is equally important. The latter are typically monitored using surrogate substrates that produce quantifiable optical signals, owing to limitations associated with "label-free" techniques that could be used to monitor the transformation of original substrate molecules. In this study, terahertz (THz) emission technology is used as a noninvasive and label-free technique to monitor the kinetics of lipase-induced hydrolysis of several substrate molecules (including the complex substrate whole cow's milk) and horseradish peroxidase-catalyzed oxidation of o-phenylenediamine in the presence of H2O2. This technique was found to be quantitative, and kinetic parameters are compared to those obtained by proton NMR spectroscopy or UV/Vis spectroscopy. This study sets the stage for investigating THz emission technology as a tool for research and development involving enzymes, and for monitoring industrial processes in the food, cosmetic, detergent, pharmaceutical, and biodiesel sectors.

リンク情報
DOI
https://doi.org/10.1002/bit.27893
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000678495900001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/bit.27893
  • ISSN : 0006-3592
  • eISSN : 1097-0290
  • Web of Science ID : WOS:000678495900001

エクスポート
BibTeX RIS