MISC

2002年9月

Novel translational control through an iron-responsive element by interaction of multifunctional protein YB-1 and IRP2

MOLECULAR AND CELLULAR BIOLOGY
  • M Ashizuka
  • ,
  • T Fukuda
  • ,
  • T Nakamura
  • ,
  • K Shirasuna
  • ,
  • K Iwai
  • ,
  • H Izumi
  • ,
  • K Kohno
  • ,
  • M Kuwano
  • ,
  • T Uchiumi

22
18
開始ページ
6375
終了ページ
6383
記述言語
英語
掲載種別
DOI
10.1128/MCB.22.18.6375-6383.2002
出版者・発行元
AMER SOC MICROBIOLOGY

The eukaryotic Y-box-binding protein YB-1 functions in various biological processes, including DNA repair, cell proliferation, and transcriptional and translational controls. To gain further insight into how human YB-1 plays its role in pleiotropic functions, we here used two-hybrid screenings to identify partners of this protein; the results showed that YB-1 itself, iron-regulatory protein 2 (IRP2), and five ribosomal proteins each served as partners to YB-1. We then examined the biological effect of the interaction of YB-1 and IRP2 on translational regulation. Both in vitro binding and coimmunoprecipitation assays showed the direct interaction of YB-1 and IRP2 in the presence of a high concentration of iron. RNA gel shift assays showed that YB-1 reduced the formation of the IRP2-mRNA complex when the iron-responsive element of the ferritin mRNA 5' untranslated region (UTR) was used as a probe. By using an in vitro translation assay using luciferase mRNA ligated to the ferritin mRNA 5'UTR as a reporter construct, we showed that both YB-1 and IRP2 inhibited the translation of the mRNA. However, coadministration of YB-1 and IRP2 proteins abrogated the inhibition of protein synthesis by each protein. An In vivo coimmunoprecipitation assay showed that IRP2 bound to YB-1 in the presence of iron and a proteasome inhibitor. The direct interaction of YB-1 and IRP2 provides the first evidence of the involvement of YB-1 in the translational regulation of an iron-related protein.

Web of Science ® 被引用回数 : 46

リンク情報
DOI
https://doi.org/10.1128/MCB.22.18.6375-6383.2002
CiNii Articles
http://ci.nii.ac.jp/naid/80015485934
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12192037
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000177642900006&DestApp=WOS_CPL