MISC

2006年12月

YB-1 is important for an early stage embryonic development - Neural tube formation and cell proliferation

JOURNAL OF BIOLOGICAL CHEMISTRY
  • Takeshi Uchiumi
  • ,
  • Abbas Fotovati
  • ,
  • Takakazu Sasaguri
  • ,
  • Kohtaro Shibahara
  • ,
  • Tatsuo Shimada
  • ,
  • Takao Fukuda
  • ,
  • Takanori Nakamura
  • ,
  • Hiroto Izumi
  • ,
  • Teruhisa Tsuzuki
  • ,
  • Michihiko Kuwano
  • ,
  • Kimitoshi Kohno

281
52
開始ページ
40440
終了ページ
40449
記述言語
英語
掲載種別
DOI
10.1074/jbc.M605948200
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

The eukaryotic Y-box- binding protein-1 (YB-1) is involved in the transcriptional and translational control of many biological processes, including cell proliferation. In clinical studies, the cellular level of YB-1 closely correlates with tumor growth and prognosis. To understand the role of YB-1 in vivo, especially in the developmental process, we generated YB-1 knock- out mice, which are embryonic lethal and exhibit exencephaly associated with abnormal patterns of cell proliferation within the neuroepithelium. beta- Actin expression and F-actin formation were reduced in the YB-1 null embryo and YB-1(-/-) mouse embryonic fibroblasts, suggesting that the neural tube defect is caused by abnormal cell morphology and actin assembly within the neuroepithelium. Fibroblasts derived from YB-1(-/-) embryos demonstrated reduced growth and cell density. A colony formation assay showed that YB-1(-/-) mouse embryonic fibroblasts failed to undergo morphological transformation and remained contact-inhibited in culture. These results demonstrate that YB-1 is involved in early mouse development, including neural tube closure and cell proliferation.

Web of Science ® 被引用回数 : 62

リンク情報
DOI
https://doi.org/10.1074/jbc.M605948200
CiNii Articles
http://ci.nii.ac.jp/naid/80018692215
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17082189
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000243033900074&DestApp=WOS_CPL