MISC

2004年4月

Targeted disruption of one allele of the Y-box binding protein-1 (YB-1) gene in mouse embryonic stem cells and increased sensitivity to cisplatin and mitomycin C

CANCER SCIENCE
  • K Shibahara
  • ,
  • T Uchiumi
  • ,
  • T Fukuda
  • ,
  • S Kura
  • ,
  • Y Tominaga
  • ,
  • Y Maehara
  • ,
  • K Kohno
  • ,
  • Y Nakabeppu
  • ,
  • T Tsuzuki
  • ,
  • M Kuwano

95
4
開始ページ
348
終了ページ
353
記述言語
英語
掲載種別
DOI
10.1111/j.1349-7006.2004.tb03214.x
出版者・発行元
JAPANESE CANCER ASSOC

The eukaryotic Y-box binding protein-1 (YB-1) functions in various biological processes, including transcriptional and translational control, DNA repair, drug resistance, and cell proliferation. To elucidate the physiological role of the YB-1 protein, we disrupted one allele of mouse YB-1 in embryonic stem (ES) cells. Northern blot analysis revealed that YB-1(+/-) ES cells with one intact allele contain approximately one-half the amount of mRNA detected in wild-type (YB-1(+/+)) cells. We further found that the protein level of YB-1(+/-) cells was reduced to approximately 50-60% compared with that of YB-1(+/+) cells. However, no apparent growth difference was found between YB-1(+/-) and YB-1(+/+) cells. YB-1(+/-) cells showed increased sensitivity to cisplatin and mitomycin C, but not to etoposide, X-ray or UV irradiation, as compared to YB-1(+/+) cells. YB-1 may have the capacity to exert a protective role against cytotoxic effects of DNA damaging agents, and may be involved in certain aspects of drug resistance.

Web of Science ® 被引用回数 : 33

リンク情報
DOI
https://doi.org/10.1111/j.1349-7006.2004.tb03214.x
CiNii Articles
http://ci.nii.ac.jp/naid/80016564909
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15072594
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000220874300011&DestApp=WOS_CPL