Apr, 2004
Targeted disruption of one allele of the Y-box binding protein-1 (YB-1) gene in mouse embryonic stem cells and increased sensitivity to cisplatin and mitomycin C
CANCER SCIENCE
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- Volume
- 95
- Number
- 4
- First page
- 348
- Last page
- 353
- Language
- English
- Publishing type
- DOI
- 10.1111/j.1349-7006.2004.tb03214.x
- Publisher
- JAPANESE CANCER ASSOC
The eukaryotic Y-box binding protein-1 (YB-1) functions in various biological processes, including transcriptional and translational control, DNA repair, drug resistance, and cell proliferation. To elucidate the physiological role of the YB-1 protein, we disrupted one allele of mouse YB-1 in embryonic stem (ES) cells. Northern blot analysis revealed that YB-1(+/-) ES cells with one intact allele contain approximately one-half the amount of mRNA detected in wild-type (YB-1(+/+)) cells. We further found that the protein level of YB-1(+/-) cells was reduced to approximately 50-60% compared with that of YB-1(+/+) cells. However, no apparent growth difference was found between YB-1(+/-) and YB-1(+/+) cells. YB-1(+/-) cells showed increased sensitivity to cisplatin and mitomycin C, but not to etoposide, X-ray or UV irradiation, as compared to YB-1(+/+) cells. YB-1 may have the capacity to exert a protective role against cytotoxic effects of DNA damaging agents, and may be involved in certain aspects of drug resistance.
- Link information
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- DOI
- https://doi.org/10.1111/j.1349-7006.2004.tb03214.x
- CiNii Articles
- http://ci.nii.ac.jp/naid/80016564909
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/15072594
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000220874300011&DestApp=WOS_CPL
- ID information
-
- DOI : 10.1111/j.1349-7006.2004.tb03214.x
- ISSN : 1347-9032
- CiNii Articles ID : 80016564909
- Pubmed ID : 15072594
- Web of Science ID : WOS:000220874300011