2003年3月
Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase
BRITISH JOURNAL OF HAEMATOLOGY
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- 巻
- 120
- 号
- 5
- 開始ページ
- 846
- 終了ページ
- 849
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1046/j.1365-2141.2003.04217.x
- 出版者・発行元
- WILEY-BLACKWELL
To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years; parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar, had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34(+) haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.
- リンク情報
- ID情報
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- DOI : 10.1046/j.1365-2141.2003.04217.x
- ISSN : 0007-1048
- PubMed ID : 12614220
- Web of Science ID : WOS:000181330200019