2017年1月
Microfluidic transmission surface plasmon resonance enhancement for biosensor applications
JAPANESE JOURNAL OF APPLIED PHYSICS
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- 巻
- 56
- 号
- 1
- 開始ページ
- 017002
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.7567/JJAP.56.017002
- 出版者・発行元
- IOP PUBLISHING LTD
The microfluidic transmission surface plasmon resonance (MTSPR) constructed by assembling a gold-coated grating substrate with a microchannel was employed for biosensor application. The transmission surface plasmon resonance spectrum obtained from the MTSPR sensor chip showed a strong and narrow surface plasmon resonance (SPR) peak located between 650 and 800 nm. The maximum SPR excitation was observed at an incident angle of 35 degrees. The MTSPR sensor chip was employed for glucose sensor application. Gold-coated grating substrates were functionalized using 3-mercapto-1-propanesulfonic acid sodium salt and subsequently functionalized using a five-bilayer poly(allylamine hydrochloride)/ poly(sodium 4-styrenesulfonate) to facilitate the coupling/ decoupling of the surface plasmon and to prepare a uniform surface for sensing. The detection limit of our developed system for glucose was 2.31 mM. This practical platform represents a high possibility of further developing several biomolecules, multiplex systems, and a point-of-care assay for practical biosensor applications. (C) 2017 The Japan Society of Applied Physics
- リンク情報
- ID情報
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- DOI : 10.7567/JJAP.56.017002
- ISSN : 0021-4922
- eISSN : 1347-4065
- Web of Science ID : WOS:000391569800001