論文

査読有り
2016年

Endothelium-Dependent and -Independent Vasodilator Effects of Dimethyl Sulfoxide in Rat Aorta

PHARMACOLOGY
  • Takeharu Kaneda
  • ,
  • Noriyasu Sasaki
  • ,
  • Norimoto Urakawa
  • ,
  • Kazumasa Shimizu

97
3-4
開始ページ
171
終了ページ
176
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1159/000443894
出版者・発行元
KARGER

This study examined the mechanism of vasorelaxation induced by dimethyl sulfoxide (DMSO) in endothelium-intact and -denuded rat aorta. DMSO (0.1-3%) inhibited phenylephrine (PE, 1 mu mol/l)-induced contraction in a dose-dependent manner. However, this relaxation was lower in the absence of the endothelium. Increase in DMSO-induced relaxation in the presence of the endothelium was attenuated by preincubation in L-N-G-nitroarginine methyl ester (L-NAME, 100 mu mol/l) and by the removal of the endothelium. In the aorta with endothelium, DMSO (3%) and CCh (3 mu mol/l) increased cGMP contents, significantly and L-NAME (100 mu mol/l) inhibited the DMSO-induced increases of cGMP. In fura 2-loaded endothelium-denuded aorta, cumulative application of DMSO (1-3%) inhibited PE-induced muscle tension; however, this application did not affect the [Ca2+](i) level. In PE-precontracted endothelium-denuded aorta, relaxation responses to fasudil were significantly less in the presence of DMSO compared to the control. These results suggest that DMSO causes relaxation by increasing the cGMP content in correlation with the release of NO from endothelial cells and by decreasing the Ca2+ sensitivity of contractile elements partly via inhibiting Rho-kinase in rat aorta. (C) 2016 S. Karger AG, Basel

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1159/000443894
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26836124
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000371351400010&DestApp=WOS_CPL