Kenji Osafune

J-GLOBAL         Last updated: Nov 12, 2019 at 17:15
 

Research Interests

 
 

Research Areas

 
 

Academic & Professional Experience

 
 
   
 
Lecturer, Kyoto University Center for iPS Cell Research and Application, Institute for Integrated Cell-Material Sciences, Kyoto University
 

Published Papers

 
Minami T, Ishii T, Yasuchika K, Fukumitsu K, Ogiso S, Miyauchi Y, Kojima H, Kawai T, Yamaoka R, Oshima Y, Kawamoto H, Kotaka M, Yasuda K, Osafune K, Uemoto S.
Regenerative Therapy   10(-) 127-133   Mar 2019   [Refereed]
Toyoda T, Kimura A, Tanaka H, Osafune K
Journal of Visualized Experiments   145    Mar 2019   [Refereed]
Matsui S, Ochiai M, Yasuda K, Mae SI, Kotaka M, Toyoda T, Yamamoto T, Osafune K
Stem Cell Research   35 101400   Mar 2019   [Refereed]
Mae SI, Ryosaka M, Osafune K
Methods in Molecular Biology   1926 117-123   Feb 2019   [Refereed]
Karagiannis P, Takahashi K, Saito M, Yoshida Y, Okita K, Watanabe A, Inoue H, Yamashita JK, Todani M, Nakagawa M, Osawa M, Yashiro Y, Yamanaka S, Osafune K
Physiological Reviews   99(1) 79-114   Jan 2019   [Refereed]
Hoshina A, Kawamoto T, Sueta SI, Mae SI, Araoka T, Tanaka H, Sato Y, Yamagishi Y, Osafune K
Scientific Reports   8(1) 6375   Apr 2018   [Refereed]
iPSC technology-based regenerative therapy for diabetes.
Kondo Y, Toyoda T, Inagaki N, Osafune K
Journal of Diabetes Investigation.   9(2) 234-243   Mar 2018   [Refereed]
Mae SI, Ryosaka M, Toyoda T, Matsuse K, Oshima Y, Tsujimoto H, Okumura S, Shibasaki A, Osafune K
495(1) 954-961   Jan 2018   [Refereed]
Adrenergic receptor agonists induce the differentiation of pluripotent stem cell-derived hepatoblasts into hepatocyte-like cells.
Kotaka M, Toyoda T, Yasuda K, Kitano Y, Okada C, Ohta A, Watanabe A, Uesugi M, Osafune K.
Scientific Reports.   7(1) 16734   Dec 2017   [Refereed]
Significance of dopamine D1 receptor signalling for steroidogenic differentiation of human induced pluripotent stem cells
Matsuo K, Sone M, Honda-Kohmo K, Toyohara T, Sonoyama T, Taura D, Kojima K, Fukuda Y, Ohno Y, Inoue M, Ohta A, Osafune K, Nakao K, Inagaki N
Scientific Reports   7(1) 15120   Nov 2017   [Refereed]
Human pluripotent stem cell-derived erythropoietin-producing cells ameliorate renal anemia in mice
Hitomi H, Kasahara T, Katagiri N, Hoshina A, Mae SI, Kotaka M, Toyohara T, Rahman A, Nakano D, Niwa A, Saito MK, Nakahata T, Nishiyama A, Osafune K
9 409   Sep 2017   [Refereed]
Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells
Kondo Y, Toyoda T, Ito R, Funato M, Hosokawa Y, Matsui S, Sudo T, Nakamura M, Okada C, Zhuang X, Watanabe A, Ohta A, Inagaki N and Osafune K
60(8) 1454-1466   Aug 2017   [Refereed]
Rho-associated kinases and non-muscle myosin IIs inhibit the differentiation of human iPSCs to pancreatic endoderm cells
Toyoda T, Kimura A, Tanaka H, Ameku T, Mima A, Hirose Y, Nakamura M, Watanabe A, and Osafune K
9(2) 419-428   Aug 2017   [Refereed]
Small molecule AT7867 proliferates PDX1-expressing pancreatic progenitor cells derived from human pluripotent stem cells
Kimura A, Toyoda T, Nishi Y, Nasu M, Ohta A and Osafune K
24 61-68   Aug 2017   [Refereed]
Modelling urea-cycle disorder citrullinemia type 1 with disease-specific iPSCs
Yoshitoshi-Uebayashi EY, Toyoda T, Yasuda K, Kotaka M, Nomoto K, Okita K, Yasuchika K, Okamoto S, Takubo N, Nishikubo T, Soga T, Uemoto S, Osafune K
486(3) 613-619   May 2017   [Refereed]
Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stem cells
Matsuno K, Mae SI, Okada C, Nakamura M, Watanabe A, Toyoda T, Uchida E, Osafune K
92(5) 281-290   Dec 2016   [Refereed]
Transcriptional Analysis of Intravenous Immunoglobulin Resistance in Kawasaki Disease Using an Induced Pluripotent Stem Cell Disease Model
Ikeda K, Mizoro Y, Ameku T, Nomiya Y, Mae SI, Matsui S, Kuchitsu Y, Suzuki C, Hamaoka-Okamoto A, Yahata T, Sone M, Okita K, Watanabe A, Osafune K, Hamaoka K
81(1) 110-118   Nov 2016   [Refereed]
Novel Regenerative Therapy for Acute Kidney Injury
Toyohara T, Osafune K
2 34   Aug 2016   [Refereed]
Identification of MMP1 as a novel risk factor for intracranial aneurysms in ADPKD using iPSC models
Ameku T, Taura D, Sone M, Numata T, Nakamura M, Shiota F, Toyoda T, Matsui S, Araoka T, Yasuno T, Mae SI, Kobayashi H, Kondo N, Kitaoka F, Amano N, Arai S, Ichisaka T, Matsuura N, Inoue S, Yamamoto T, Takahashi K, Asaka I, Yamada Y, Ubara Y, Muso E, Fukatsu A, Watanabe A, Sato Y, Nakahata T, Mori Y, Koizumi A, Nakao K, Yamanaka S & Osafune K
60 30013   Jul 2016   [Refereed]
Cell therapy using human induced pluripotent stem cell-derived renal progenitors ameliorates acute kidney injury in mice
Toyohara T, Mae SI, Sueta SI, Inoue T, Yamagishi Y, Kawamoto T, Kasahara T, Hoshina A, Toyoda T, Tanaka H, Araoka T, Sato-Otsubo A, Takahashi K, Sato Y, Yamaji N, Ogawa S, Yamanaka S, Osafune K
4(9) 980-992   Sep 2015   [Refereed]
Directing the differentiation of pluripotent stem cells to renal endpoints.
Kenji Osafune
Kidney Development, Repair and Regeneration   473-490   Sep 2015   [Refereed]
Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches
Miki K, Endo K, Takahashi S, Funakoshi S, Takei I, Katayama S, Toyoda T, Kotaka M, Takaki T, Umeda M, Okubo C, Nishikawa M, Oishi A, Narita M, Miyashita I, Asano K, Hayashi K, Osafune K, Yamanaka S, Saito H, Yoshida Y
16(6) 699-711   Jul 2015   [Refereed]
Vascularized and complex organ buds generated from diverse tissues via mesenchymal cell-driven condensation.
Takebe T, Enomura M, Yoshizawa E, Kimura M, Koike H, Matsuzaki T, Yamazaki T, Toyohara T, Osafune K, Nakauchi H, Yoshikawa HY, Taniguchi H
16(5) 556-565   May 2015   [Refereed]
Kidney regeneration from human iPSCs
Mae S, Osafune K
20(2) 171-177   Apr 2015   [Refereed]
Cell aggregation optimizes the differentiation of human ESCs and iPSCs into pancreatic bud-like progenitor cells.
Toyoda T, Mae S, Tanaka H, Kondo Y, Funato M, Hosokawa Y, Sudo T, Kawaguchi Y, Osafune K
14(2) 185-197   Mar 2015   [Refereed]
Generation of Alveolar Epithelial Spheroids via Isolated Progenitor Cells from Human Pluripotent Stem Cells
Gotoh S, Ito I, Nagasaki T, Yamamoto Y, Konishi S, Korogi Y, Matsumoto H, Muro S, Hirai T, Funato M, Mae S, Toyoda T, Sato-Otsubo A, Ogawa S, Osafune K, Mishima M
3(3) 394-403   Sep 2014   [Refereed]
Functional analysis of iPSC-derived myocytes from a patient with carnitine palmitoyltransferase II deficiency
Yasuno T, Osafune K, Sakurai H, Asaka I, Tanaka A, Yamaguchi S, Yamada K, Hitomi H, Arai S, Kurose Y, Higaki Y, Sudo M, Ando S, Nakashima H, Saito T, Kaneoka H
448(2) 175-181   May 2014   [Refereed]
Cell therapy for kidney injury: different options and mechanisms – Kidney progenitor cells
Osafune K
126(2) 64   May 2014   [Refereed]
Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm
Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, Nakamura M, Sutou K, Osafune K, Yamanaka S
5 3678   Apr 2014   [Refereed]
K. Ohnishi; K. Semi; T. Yamamoto; M. Shimizu; A. Tanaka; K. Mitsunaga; K. Okita; K. Osafune; Y. Arioka; T. Maeda; H. Soejima; H. Moriwaki; S. Yamanaka; K. Woltjen; Y. Yamada
Cell   156(4) 663-677   Feb 2014   [Refereed]
R. Inoue; K. Asanuma; T. Seki; M. Nagase; K. Osafune
Folia Pharmacologica Japonica   143(1) 27-33   Jan 2014   [Refereed]
Efficient and rapid induction of human iPSCs/ESCs into nephrogenic intermediate mesoderm using small molecule-based differentiation methods
Araoka T, Mae S, Kurose Y, Uesugi M, Ohta A, Yamanaka S, Osafune K
9(1) e84881   Jan 2014   [Refereed]
M. Nakagawa; Y. Taniguchi; S. Senda; N. Takizawa; T. Ichisaka; K. Asano; A. Morizane; D. Doi; J. Takahashi; M. Nishizawa; Y. Yoshida; T. Toyoda; K. Osafune; K. Sekiguchi; S. Yamanaka
Scientific Reports   4 3594   Jan 2014   [Refereed]
K. Osafune
Regenerative Medicine   9(1) 9-12   Jan 2014   [Refereed]
T. Hitomi; T. Habu; H. Kobayashi; H. Okuda; K.H. Harada; K. Osafune; D. Taura; M. Sone; I. Asaka; T. Ameku; A. Watanabe; T. Kasahara; T. Sudo; F. Shiota; H. Hashikata; Y. Takagi; D. Morito; S. Miyamoto; K. Nakao; A. Koizumi
Biochemical and Biophysical Research Communications   439(4) 419-426   Oct 2013   [Refereed]
T. Hitomi; T. Habu; H. Kobayashi; H. Okuda; K.H. Harada; K. Osafune; D. Taura; M. Sone; I. Asaka; T. Ameku; A. Watanabe; T. Kasahara; T. Sudo; F. Shiota; H. Hashikata; Y. Takagi; D. Morito; S. Miyamoto; K. Nakao; A. Koizumi
Biochemical and Biophysical Research Communications   438(1) 13-19   Aug 2013   [Refereed]
Downregulation of Securin by the variant RNF213 R4810K reduces angiogenic activity of induced pluripotent stem cell-derived vascular endothelial cells from moyamoya patients.
Hitomi T, Habu T, Kobayashi H, Okuda H, Harada KH, Osafune K, Taura D, Sone M, Asaka I, Ameku T, Watanabe A, Kasahara T, Sudo T, Shiota F, Hashikata H, Takagi Y, Morito D, Miyamoto S, Nakao K, Koizumi A
438(1) 13-19   Aug 2013   [Refereed]
A. Nasu; M. Ikeya; T. Yamamoto; A. Watanabe; Y. Jin; Y. Matsumoto; K. Hayakawa; N. Amano; S. Sato; K. Osafune; T. Aoyama; T. Nakamura; T. Kato; J. Toguchida
PLoS ONE   8(1) e53771   Jan 2013   [Refereed]
Mae SI, Shono A, Shiota F, Yasuno T, Kajiwara M, Gotoda-Nishimura N, Arai S, Sato-Otubo A, Toyoda T, Takahashi K, Nakayama N, Cowan CA, Aoi T, Ogawa S, McMahon AP, Yamanaka S, Osafune K.
Nat.Commun.   4 1367   Jan 2013   [Refereed]
[iPS cell technology-based strategies for the treatment of diabetic nephropathy].
K. Osafune
Nihon rinsho. Japanese journal of clinical medicine   70 Suppl 5 419-423   2012   [Refereed]
K. Osafune
Seminars in Nephrology   32(5) 479-485   Sep 2012   [Refereed]
K. Osafune; S. Yamanaka
Regenerative Nephrology   203-215   2011   [Refereed]
K. Osafune
Experimental Cell Research   316(16) 2571-2577   Oct 2010   [Refereed]
Osafune K and Yamanaka S
Regenerative nephrology   203-15   2010   [Refereed]
Induced pluripotent stem (iPS) cells: an up-to-the-minute review. 
Lau F, Ahfeldt T, Osafune K, Akutsu H, Cowan CA.
F1000 Biology Reports 1: 84, 2009.   1 84   Nov 2009   [Refereed]
A small molecule that directs differentiation of human embryonic stem cells into the pancreatic lineage
Chen S, Borowiak M, Fox J, Maehr R, Osafune K, Davidow L, Lam K, Peng L, Schreiber S, Rubin L, Melton DA
5(4) 258-265   Apr 2009   [Refereed]
S. Chen; M. Borowiak; J.L. Fox; R. Maehr; K. Osafune; L. Davidow; K. Lam; L.F. Peng; S.L. Schreiber; L.L. Rubin; D. Melton
Nature Chemical Biology   5(4) 258-265   2009   [Refereed]
D. Huangfu; K. Osafune; R. Maehr; W. Guo; A. Eijkelenboom; S. Chen; W. Muhlestein; D.A. Melton
Nature Biotechnology   26(11) 1269-1275   Nov 2008   [Refereed]
K. Osafune; L. Caron; M. Borowiak; R.J. Martinez; C.S. Fitz-Gerald; Y. Sato; C.A. Cowan; K.R. Chien; D.A. Melton
Nature Biotechnology   26(3) 313-315   Mar 2008   [Refereed]
K. Osafune; A.E. Chen; D.A. Melton
Human Embryonic Stem Cells: The Practical Handbook   179-186   2007   [Refereed]
R. Nishinakamura; K. Osafune
Journal of Physiological Sciences   56(2) 131-136   Apr 2006   [Refereed]
H. Kanamori; M. Ota; H. Takeoka; K. Osafune; S. Yonezu; A. Fukatsu; K. Kanatsu
Clinical and Experimental Nephrology   10(1) 68-73   Mar 2006   [Refereed]
K. Osafune; M. Takasato; A. Kispert; M. Asashima; R. Nishinakamura
Development   133(1) 151-161   Jan 2006   [Refereed]
M. Takasato; K. Osafune; Y. Matsumoto; Y. Kataoka; N. Yoshida; H. Meguro; H. Aburatani; M. Asashima; R. Nishinakamura
Mechanisms of Development   121(6) 547-557   Jun 2004   [Refereed]
K. Osafune; R. Nishinakamura; S. Komazaki
Development Growth and Differentiation   44(2) 161-167   Apr 2002   [Refereed]
K. Osafune; N. Iehara; H. Takeoka; T. Doi; T. Kita
Journal of Internal Medicine   247(2) 299-300   Feb 2000   [Refereed]
K. Osafune; T. Yoshinaga; M. Tadano; H. Takeoka; Y. Kageyama; R. Mimura; H. Saita; K. Ichikawa; K. Kanatsu
Clinical and Experimental Nephrology   4(4) 348-351   Dec 2000   [Refereed]
K. Osafune; H. Takeoka; H. Kanamori; H. Koshiyama; K. Hirose; M. Hanada; R. Mimura; F. Ando; K. Kanatsu
Clinical and Experimental Nephrology   4(4) 329-334.   Dec 2000   [Refereed]
K. Osafune; K. Ichikawa; T. Yasui; A. Sekikawa; H. Takeoka; K. Kanatsu; K. Kohigashi; H. Koshiyama
Internal Medicine   38(7) 590-596   Jul 1999   [Refereed]

Research Grants & Projects

 
Development of regenerative medicine for kidney disease with iPS cell
JST Basic Research Programs (Precursory Research for Embryonic Science and Technology :PRESTO)
Project Year: 2008 - 2012