Kenji Osafune

Profile Information

Affiliation: Kyoto University

ORCID ID: https://orcid.org/0000-0001-7238-2763
J-GLOBAL ID: 200901043397093190
researchmap Member ID: 6000014252

External link: http://www.osafune.icems.kyoto-u.ac.jp/

Research Interests

Research Areas

Life sciences / Nephrology /

Research History

Apr, 2022 - Present

Department Head, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University
Oct, 2014 - Present

Professor, Center for iPS Cell Research and Application, Kyoto University
Apr, 2010 - Sep, 2014

Associate Professor, Center for iPS Cell Research and Application, Kyoto University
Jun, 2008 - Mar, 2010

PRESTO Research Fellow, Japan Science and Technology Agency
Jun, 2008 - Mar, 2010

Lecturer, Center for iPS Cell Research and Application, Institute for Integrated Cell-Material Sciences, Kyoto University
Apr, 2005 - May, 2008

Postdoctoral Fellow, Department of Stem Cells and Regenerative Biology, Harvard Stem Cell Institute, Harvard University
Apr, 2003 - Mar, 2005

Research Fellow, Graduate School and College of Arts and Sciences, the University of Tokyo
Apr, 2000 - Mar, 2003

Graduate School and College of Arts and Sciences, the University of Tokyo (Doctoral Course)
Apr, 1996 - Mar, 2000

Resident, Maizuru Municipal Hospital, Kyoto University Hospital, and Hyogo Prefectural Amagasaki Hospital
Apr, 1990 - Mar, 1996

Faculty of Medicine, Kyoto University

Papers

Development of an osteosarcoma model with MYCN amplification and TP53 mutation in hiPS cell‐derived neural crest cells

Kyosuke Mukae, Hisanori Takenobu, Yuki Endo, Masayuki Haruta, Tianyuan Shi, Shunpei Satoh, Miki Ohira, Michinori Funato, Junya Toguchida, Kenji Osafune …

Cancer Science, 114(5) 1898-1911, May, 2023 Peer-reviewed
In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor cells

Hiraku Tsujimoto, Naoko Katagiri, Yoshihiro Ijiri, Ben Sasaki, Yoshifumi Kobayashi, Akira Mima, Makoto Ryosaka, Kenichiro Furuyama, Yoshiya Kawaguchi, Kenji Osafune

PLOS ONE, 17(11) e0275600-e0275600, Nov 15, 2022 Peer-reviewed
Podocytes are lost from glomeruli before completing apoptosis

Kazuyoshi Yamamoto, Masahiro Okabe, Keiko Tanaka, Takashi Yokoo, Ira Pastan, Toshikazu Araoka, Kenji Osafune, Tomohiro Udagawa, Masahiro Koizumi, Taiji Matsusaka

American Journal of Physiology-Renal Physiology, 323(5) F515-F526, Nov 1, 2022 Peer-reviewed
Purification of human iPSC-derived cells at large scale using microRNA switch and magnetic-activated cell sorting.

Yuta Tsujisaka, Takeshi Hatani, Chikako Okubo, Ryo Ito, Azuma Kimura, Megumi Narita, Kazuhisa Chonabayashi, Shunsuke Funakoshi, Antonio Lucena-Cacace, Taro Toyoda …

Stem cell reports, 17(7) 1772-1785, Jul 12, 2022
Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids

Makoto Ryosaka, Shin-Ichi Mae, Kenji Osafune

STAR Protocols, 3(3) 101484-101484, Jun 17, 2022 Peer-reviewed
Current status and future directions of clinical applications using iPS cells - focus on Japan

Tsujimoto H, Osafune K

The FEBS journal, Aug 18, 2021 Peer-reviewed
iPSC technology-based regenerative medicine for kidney diseases

Osafune K

Clinical and Experimental Nephrology, 25(6) 574-584, Jun, 2021 Peer-reviewed
Small molecule TCS21311 can replace BMP7 and facilitate cell proliferation in in vitro expansion culture of nephron progenitor cells

Tsujimoto H, Araoka T, Nishi Y, Ohta A, Nakahata T, Osafune K

Biochemical and Biophysical Research Communications, 558 231-238, Jun, 2021 Peer-reviewed
Regenerative treatments for kidney diseases: the closest and fastest strategies to solving related medical and economic problems

Osafune K

Artificial Organs, 45(5) 447-453, May, 2021 Peer-reviewed
Identification of candidate PAX2-regulated genes implicated in human kidney development

Yamamura Y, Furuichi K, Murakawa Y, Hirabayashi S, Yoshihara M, Sako K, Kitajima S, Toyama T, Iwata Y, Sakai N …

Scientific Reports, 11(1) 9123, Apr 27, 2021
Feasibility of large experimental animal models in testing novel therapeutic strategies for diabetes

Nagaya M, Hasegawa K, Uchikura A, Nakano K, Watanabe M, Umeyama K, Matsunari H, Osafune K, Kobayashi E, Nakauchi H …

World Journal of Diabetes, 12(4) 306-330, Apr 15, 2021 Peer-reviewed
Kidney organoids: research in developmental biology and emerging applications

Shimizu T, Yamagata K, Osafune K

63(2) 166-177, Feb, 2021 Peer-reviewed
Retinoic acid regulates erythropoietin production cooperatively with hypoxia-inducible factors in human iPSC-derived erythropoietin-producing cells

Katagiri N, Hitomi H, Mae SI, Kotaka M, Lei L, Yamamoto T, Nishiyama A, Osafune K

Scientific Reports, 11(1) 3936-3936, Feb, 2021 Peer-reviewed
Combined Omics Approaches Reveal the Roles of Non-canonical WNT7B Signaling and YY1 in the Proliferation of Human Pancreatic Progenitor Cells.

Kimura A, Toyoda T, Iwasaki M, Hirama R, Osafune K

Cell Chemical Biology, 27(12) 1561-1572, Dec, 2020 Peer-reviewed
Genetically engineered pigs manifesting pancreatic agenesis with severe diabetes

Nagaya M, Hasegawa K, Watanabe M, Nakano K, Okamoto K, Yamada T, Uchikura A, Osafune K, Yokota H, Nagaoka T …

BMJ Open Diabetes Research & Care, 8(2) e001792, Nov, 2020
Pluripotent stem cell model of Shwachman-Diamond syndrome reveals apoptotic predisposition of hemoangiogenic progenitors.

Takayuki Hamabata, Katsutsugu Umeda, Kagehiro Kouzuki, Takayuki Tanaka, Tomoo Daifu, Seishiro Nodomi, Satoshi Saida, Itaru Kato, Shiro Baba, Hidefumi Hiramatsu …

Scientific reports, 10(1) 14859-14859, Sep, 2020 Peer-reviewed
A novel ADPKD model using kidney organoids derived from disease-specific human iPSCs

Tatsuya Shimizu, Shin-Ichi Mae, Toshikazu Araoka, Keisuke Okita, Akitsu Hotta, Kunihiro Yamagata, Kenji Osafune

Biochemical and Biophysical Research Communications, 529(4) 1186-1194, Sep, 2020 Peer-reviewed
PKD1-Dependent Renal Cystogenesis in Human Induced Pluripotent Stem Cell-Derived Ureteric Bud/Collecting Duct Organoids

Shohei Kuraoka, Shunsuke Tanigawa, Atsuhiro Taguchi, Akitsu Hotta, Hitoshi Nakazato, Kenji Osafune, Akio Kobayashi, Ryuichi Nishinakamura

Journal of the American Society of Nephrology, 31(10) 2355-2371, Aug 3, 2020 Peer-reviewed
Expansion of Human iPSC-Derived Ureteric Bud Organoids with Repeated Branching Potential

Shin-Ichi Mae, Makoto Ryosaka, Satoko Sakamoto, Kyoko Matsuse, Aya Nozaki, Maiko Igami, Ryotaro Kabai, Akira Watanabe, Kenji Osafune

Cell Reports, 32(4) 107963-107963, Jul, 2020 Peer-reviewed
Failure to confirm a sodium–glucose cotransporter 2 inhibitor‐induced hematopoietic effect in non‐diabetic rats with renal anemia

Daisuke Yamazaki, Yoshio Konishi, Takashi Morikawa, Hideki Kobara, Tsutomu Masaki, Hirofumhi Hitomi, Kenji Osafune, Daisuke Nakano, Wararat Kittikulsuth, Akira Nishiyama

Journal of Diabetes Investigation, 11(4) 834-843, Jul, 2020 Peer-reviewed

Misc.

218

腎オルガノイドを用いた多発性嚢胞腎モデル解析

清水達也, 山縣邦弘, 長船健二

腎臓内科 (科学評論社), 17(6) 642-649, Jun 28, 2023
腎オルガノイドを用いた研究展望

長船健二

腎臓内科 (科学評論社), 17(6) 627-633, Jun 28, 2023
「腎臓オルガノイド」

河野有香, 小林恭, 長船健二

Medical Science Digest (ニューサイエンス社), 49(5), Apr 21, 2023
iPS細胞

大槻健弥, 長船健二

「腎と透析」（東京医学社）, 94(3) 359-362, Mar 25, 2023
第3章応用編、第2節医療応用、iPS細胞

安田勝太郎, 長船健二

ゲノム編集技術～実験上のポイント/産業利用に向けた研究開発動向と安全性周知, 127-138, Jan 23, 2023
膵β細胞再生医療のUpdate

畑野悠, 長船健二

Diabetes Journal, 49(4) 6(127)-13(134), Oct 31, 2022
Life Science Key Notes

16-20, Oct 21, 2022
ヒトiPS細胞由来ネフロンオルガノイドを用いたADPKD腎嚢胞モデルの作製と創薬スクリーニング系の開発

清水達也, 前伸一, 荒岡利和, 山縣邦弘, 長船健二

発達腎研究会誌, 29(1) 28-33, Apr, 2022
腎臓の再生医療の現状

長船健二

ぜんじんきょう, 309 20-21, Dec, 2021
留学中および留学予定の皆さまへ－be bold !－

長船健二

Life Science Connect－研究知識と留学経験を繋ぐ－, 2 8-11, Dec, 2021
iPS細胞を用いた囊胞性疾患の研究

前伸一, 長船健二

腎臓内科, 14(5) 609-617, Nov 28, 2021
腎臓オルガノイド作製法とそれを用いた腎機能解析

豊原光佑, 長船健二

腎と透析 11月号, 91(5) 907-7912, Nov 25, 2021
腎臓再生の最前線

平山隆一郎, 長船健二

泌尿器科, 13(6) 747-755, Jun, 2021
糖尿病に対する細胞療法確立に向けたヒトES/iPS細胞由来膵前駆細胞の増殖機序の解明

木村東, 豊田太郎, 岩崎未央, 平間竜介, 長船健二

糖尿病(Web), 64(Suppl), 2021
Developmental physiology and diseases; Stem cells and kidney regeneration

Osafune K, Pleniceanu O, Dekel B

Pediatric Nephrology, 8th edition (Springer)., 2021
Stem cells in regenerative processes; Induced pluripotent stem cells

Osafune K

Regenerative Nephrology, 2nd edition (Elsevier)., 2021
iPS細胞を用いた腎疾患に対する再生医療の開発

長船健二

日本内科学会雑誌, 109(12) 2553-2561, Dec, 2020 Invited
iPS細胞を用いた腎疾患と糖尿病に対する再生医療の開発

畑野悠, 木村東, 長船健二

医学のあゆみ, 275(9) 990-996, Nov, 2020
個別に分化させた複数種の細胞を組み合わせて、腎臓オルガノイドをつくる

長船健二

ニュートン別冊, 158-159, Nov, 2020
iPS細胞を用いた腎臓、膵臓、肝臓の再生医療開発

長船健二

BIO EX-press 2020秋, 14-18, Oct, 2020 Invited

Books and Other Publications

もっとよくわかる！幹細胞と再生医療

長船健二

羊土社, Mar, 2014

Presentations

428

「iPS細胞による１型糖尿病根治」

長船健二

日本IDDMネットワークサイエンスフォーラム in 大阪 Invited
臓器再生のフロンティア「iPS 細胞を用いた腎、膵、肝疾患に対する再生医療と新規治療薬の開発」

長船健二

第 96 回日本内分泌学会学術総会特別シンポジウム3, Jun 3, 2023, 第 96 回日本内分泌学会 Invited
“Liver Regeneration Using iPSC in Liver Cirrhosis”

Osafune K

The Liver Week 2023, May 20, 2023 Invited
iPSC technology-based regenerative medicine”

Osafune K

May 19, 2023, 仁川（ガチョン）大学ギル医療センター Invited
“iPSC-based regenerative therapies for kidney diseases and diabetes”

Osafune K

A*CGT Symposium 2023, May 17, 2023 Invited
iPSC technology-based regenerative therapy and drug discovery for kidney diseases

Apr 22, 2023 Invited
肝硬変治療確立に向けたカニクイザル肝線維症モデルのエラストグラフィによる肝線維化評価の検討

安田勝太郎, 片貝祐子, 酒井克也, 揚山直英, 長船健二

第22回日本再生医療学会総会, Mar 24, 2023
ヒト多能性幹細胞由来の腎前駆細胞培養系に未分化多能性幹細胞の残存がないことを確認する方法の開発

辻本啓, 片桐直子, 井尻芳弘, 佐々木勉, 小林義史, 美馬明, 兩坂誠, 古山賢一郎, 川口義弥, 長船健二

第22回日本再生医療学会総会, Mar 24, 2023
ヒトiPS細胞由来ネフロン前駆細胞を用いたAKIおよびCKDに対する新規の細胞療法の開発

荒岡利和, 兩坂誠, 豊原光佑, 乾ちひろ, 田畑泰彦, 長船健二

第22回日本再生医療学会総会, Mar 24, 2023
ヒトiPS細胞分化モデルを用いた膵内胚葉分化におけるHHEXの役割の解明

伊藤遼, 木村東, 廣瀬友里恵, 畑野悠, 美馬淳志, 前伸一, 境内大和, 中村聡宏, 藤倉純二, 西洋平, 太田章, 豊田太郎, 稲垣暢也, 長船健二

第22回日本再生医療学会総会, Mar 24, 2023
ヒト膵β細胞の増殖促進因子の探索と増殖機構の解明

前田貴広, 木村東, 伊藤遼, 布施広光, 太田章, 長船健二

第22回日本再生医療学会総会, Mar 24, 2023
胆汁うっ滞症研究のための肝内胆管チップの開発

出口清香, 根来亮介, 小杉佳織, 小髙真希, 小川絵里, 岡本竜弥, 長船健二, 鳥澤勇介, 高山和雄

第22回日本再生医療学会総会, Mar 24, 2023
iPS細胞由来腎オルガノイドを用いた難治性腎疾患に対する創薬研究

長船健二

第22回日本再生医療学会総会, Mar 23, 2023 Invited
オルガノイドを用いた腎疾患に対する創薬研究の現状と展望

長船健二

日本製薬工業協会研究開発委員会セミナー, Mar 22, 2023, 日本製薬工業協会 Invited
Mechanistic anlayses of differentiation and proliferation of pancreatic progenitor cells derived from human iPSCs

Osafune K

Translational Advances in Stem Cells for Diabetes Symposium., Mar 8, 2023 Invited
iPS細胞を用いた腎、膵、肝疾患に対する再生医療と新規治療薬の開発

長船健二

第11回細胞再生医療研究会（甲南大学フロンティアサイエンス学部）, Mar 4, 2023 Invited
iPS細胞を用いた腎疾患に対す新規治療法の開発

長船健二

神奈川県立保健福祉大学セミナー., Dec 26, 2022 Invited
iPS細胞を用いた腎疾患に対す新規治療法の開発

長船健二

HVC KYOTOウェルネスシンポジウム～細胞バイオテクノロジーの観点から再生医療関連ビジネスを再考してみるのは面白い～, Dec 14, 2022, 日本貿易振興機構（JETRO）、京都府、京都市、京都ﾘｻｰﾁﾊﾟｰｸ（株） Invited
iPS細胞を用いた腎、膵、肝疾患に対する再生医療と新規治療薬の開発

長船健二

第12回バイオメディカル研究所インタラクティブセミナー, Dec 6, 2022, 信州大学バイオメディカル研究所 Invited
Exploration of proliferation-promoting factors for human pancreatic beta cells

Takahiro Maeda, Azuma Kimura, Ryo Ito, Kenji Osafune

Nov 30, 2022

Professional Memberships

Research Projects

ヒトiPS細胞を用いた腎臓再生医療の開発

ＪＳＴ戦略的創造研究推進制度（個人研究型）（個人研究推進事業：さきがけ研究２１‐ＰＲＥＳＴＯ）, 2008 - 2012
Development of regenerative medicine for kidney disease with iPS cell

JST Basic Research Programs (Precursory Research for Embryonic Science and Technology :PRESTO), 2008 - 2012

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