2002年
Isolation of the interacting molecules with GEX-3 by a novel functional screening
Biochemical and Biophysical Research Communications
- ,
- ,
- ,
- ,
- 巻
- 292
- 号
- 3
- 開始ページ
- 697
- 終了ページ
- 701
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1006/bbrc.2002.6717
To screen for important molecules that interact with a gene of interest in Caenorhabditis elegans (C. elegans), we established a novel functional screening system using the yeast two-hybrid system with the RNA interference technique. Our screening system makes it possible to identify the molecular machinery involved in the function of a gene of interest starting with the cDNA of this gene. As a model case, we examined the molecular machinery involved in the function of GEX-3, an essential factor of tissue morphogenesis. We identified many interacting molecules by yeast two-hybrid screening and could detect some functional interactions using this novel functional screening system. © 2002 Elsevier Science (USA).
- ID情報
-
- DOI : 10.1006/bbrc.2002.6717
- ISSN : 0006-291X
- PubMed ID : 11922622
- SCOPUS ID : 0036086109