2015年8月
Caspase-dependent drug-induced apoptosis is regulated by cell surface sialylation in human B-cell lymphoma
ONCOLOGY LETTERS
- ,
- ,
- 巻
- 10
- 号
- 2
- 開始ページ
- 687
- 終了ページ
- 690
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3892/ol.2015.3320
- 出版者・発行元
- SPANDIDOS PUBL LTD
The important role of sialic acid in various biological phenomena is well-established. In order to further clarify the role of sialic acid in cell death induced by various stimuli, the present study compared the cell survival of the HBL-2 human diffuse large B-cell lymphoma cell line upon anticancer drug-induced cell death, with or without neununinidase pretreatment: Cell survival was assessed using flow cytometry. Upon treatment with doxorubicin or ctoposide, the HBL-2 cell viability decreased. In etoposide-induced cell death, the HBL-2 cells demonstrated nuclear fragmentation, which was consistent with morphologically apoptotic cells. In addition, a higher decrease in the cell viability of ctoposide-treated HBL-2 cells was observed in cells pretreated with neuraminidase compared with cells that were not pretreated. Furthermore, the caspase-3, caspase-8 and caspase-9 activities in etoposide-induced apoptosis demonstrated a greater increase upon ricuraminidase pretreatment compared with no neuraminidase pretreatment. In conclusion, cell surface sialylation appears to protect lymphoma cells from anticancer drug-induced apoptosis.
- リンク情報
- ID情報
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- DOI : 10.3892/ol.2015.3320
- ISSN : 1792-1074
- eISSN : 1792-1082
- Web of Science ID : WOS:000358676200020