論文

査読有り
2007年5月

Exercise-induced activation of cardiac sympathetic nerve triggers cardioprotection via redox-sensitive activation of eNOS and upregulation of iNOS

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
  • Yuzo Akita
  • ,
  • Hajime Otani
  • ,
  • Seiji Matsuhisa
  • ,
  • Shiori Kyoi
  • ,
  • Chiharu Enoki
  • ,
  • Reiji Hattori
  • ,
  • Hiroji Imamura
  • ,
  • Hiroshi Kamihata
  • ,
  • Yutaka Kimura
  • ,
  • Toshiji Iwasaka

292
5
開始ページ
H2051
終了ページ
H2059
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1152/ajpheart.01102.2006
出版者・発行元
AMER PHYSIOLOGICAL SOC

We investigated the mechanism of exercise-induced late cardioprotection against ischemia-reperfusion (I/R) injury. C57BL/6 mice received treadmill exercise (60 min/day) for 7 days at a work rate of 60-70% maximal oxygen uptake. Exercise transiently increased oxidative stress and activated endothelial isoform of nitric oxide synthase (eNOS) during exercise and increased expression of inducible isoform of NOS (iNOS) in the heart after 7 days of exercise. The mice were subjected to regional ischemia by 30 min of occlusion of the left coronary artery, followed by 2 h of reperfusion. Infarct size was significantly smaller in the exercised mice. Ablation of cardiac sympathetic nerve by topical application of phenol abolished oxidative stress, activation of eNOS, upregulation of iNOS, and cardioprotection mediated by exercise. Treatment with the antioxidant N-(2-mercaptopropionyl)-glycine during exercise also inhibited activation of eNOS, upregulation of iNOS, and cardioprotection. fit eNOS(-/-) mice exercise-induced oxidative stress was conserved, but upregulation of iNOS and cardioprotection was lost. Exercise did not confer cardioprotection when the iNOS selective inhibitor 1400W was administered just before coronary artery Occlusion or when iNOS(-/-) mice were employed. These results suggest that exercise stimulates cardiac sympathetic nerves that provoke redox-sensitive activation of eNOS, leading to upregulation of iNOS, which acts as a mediator of late cardioprotection against I/R injury.

リンク情報
DOI
https://doi.org/10.1152/ajpheart.01102.2006
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000247777200006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1152/ajpheart.01102.2006
  • ISSN : 0363-6135
  • Web of Science ID : WOS:000247777200006

エクスポート
BibTeX RIS