論文

査読有り 国際誌
2019年7月

LGR4 is essential for R-spondin1-mediated suppression of food intake via pro-opiomelanocortin.

Bioscience, biotechnology, and biochemistry
  • Ayano Otsuka
  • ,
  • Ayana Jinguji
  • ,
  • Yuko Maejima
  • ,
  • Yoshiyuki Kasahara
  • ,
  • Kenju Shimomura
  • ,
  • Shizu Hidema
  • ,
  • Katsuhiko Nishimori

83
7
開始ページ
1336
終了ページ
1342
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2019.1591266

Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4) suppresses food intake after its activation by binding of its ligands, R-spondins. We investigated the mechanism of food intake suppression by R-spondin1 in a region-specific Lgr4 gene knockout (LGR4 cKO) mouse model, generated by deletion of the Lgr4 gene in arcuate nucleus (ARC) using Lgr4fx/fx mice combined with infection of an AAV-Cre vector. After R-spondin1 administration, LGR4 cKO mice didn't exhibit a suppressed appetite, compared to that in control mice, which received a vehicle. In ARC of LGR4 cKO mice, Pomc mRNA expression was reduced, leading to suppressed food intake. On the other hand, neurons-specific LGR4 KO mice exhibited no differences in Pomc expression, and no structural differences were observed in the ARC of mutant mice. These results suggest that LGR4 is an essential part of the mechanism, inducing Pomc gene expression with R-spondin1 in ARC neurons in mice, thereby regulating feeding behavior. Abbreviations: LGR4: Leucine-rich repeat-containing G-protein coupled receptor 4; RSPOs: roof plate-specific spondins; ARC: arcuate nucleus; AAV: adeno associated virus; POMC: pro-opiomelanocortin; CART: cocaine and amphetamine-regulated transcript; NPY: neuropeptide Y; AgRP: agouti-related peptide; Axin2: axis inhibition protein 2; Lef1: lymphoid enhancer binding factor 1; ccnd1: cyclin D1.

リンク情報
DOI
https://doi.org/10.1080/09168451.2019.1591266
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30916623
ID情報
  • DOI : 10.1080/09168451.2019.1591266
  • ISSN : 0916-8451
  • PubMed ID : 30916623

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