2014年5月
Role of En2 in the tectal laminar formation of chick embryos
DEVELOPMENT
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- 巻
- 141
- 号
- 10
- 開始ページ
- 2131
- 終了ページ
- 2138
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1242/dev.102905
- 出版者・発行元
- COMPANY OF BIOLOGISTS LTD
The chick optic tectum consists of 16 laminae. Here, we report contribution of En2 to laminar formation in chick optic tecta. En2 is specifically expressed in laminae g-j of stratum griseum et fibrosum superficiale (SGFS). Misexpression of En2 resulted in disappearance of En2-expressing cells from the superficial layers (laminae a-f of SGFS), where endogenous En2 is not expressed. Misexpression of En2 before postmitotic cells had left the ventricular layer indicated that En2-misexpressing cells stopped at the laminae of endogenous En2 expression and that they did not migrate into the superficial layers. Induction of En2 misexpression using a tetracycline-inducible system after the postmitotic cells had reached superficial layers also resulted in disappearance of En2-expressing cells from the superficial layers. Time-lapse analysis showed that En2-misexpressing cells migrated back from the superficial layers towards the middle layers, where En2 is strongly expressed endogenously. Our results suggest a potential role of En2 in regulating cell migration and positioning in the tectal laminar formation.
- リンク情報
- ID情報
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- DOI : 10.1242/dev.102905
- ISSN : 0950-1991
- eISSN : 1477-9129
- PubMed ID : 24803658
- Web of Science ID : WOS:000336126400015