1997年7月
Study on phosphate uptake of the marine cyanophyte Synechococcus sp NIBB 1071 in relation to oligotrophic environments in the open ocean
MARINE BIOLOGY
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- ,
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- 巻
- 129
- 号
- 1
- 開始ページ
- 195
- 終了ページ
- 202
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s002270050160
- 出版者・発行元
- SPRINGER
Inorganic phosphate (Pi) uptake by the marine cyanophyte Synechococcus sp. NIBB 1071 was studied using cells grown in an artificial seawater medium. The phosphate uptake was markedly enhanced in cells grown in the medium of low phosphate concentrations (phosphate-limited cells) than in cells grown in the phosphate-rich medium (phosphate-replete cells). The diagnosis of kinetics of instantaneous phosphate-uptake showed that V-max of the former was more than two orders of magnitude greater than that of the latter, and the k(m) of the former was about 1/20 of that of the latter. The enhancement of the phosphate uptake was completed after a 40-h incubation of phosphate-replete cells in the phosphate-free medium. The activation was suppressed by chloramphenicol, an inhibitor of protein synthesis. The uptake developed in phosphate-limited cells was energy dependent and susceptive to osmotic shock, which suggests the involvement of a periplasmic phosphate-binding protein, analogous to that found in heterotrophic gram-negative eubacterial cells. The relationship between phosphate quota and growth rate, together with the kinetical data for phosphate uptake, predicted that ambient phosphate as low as 0.5 nM could support cell growth at a rate of one division per day. Results indicate that cells can grow rapidly even at phosphate concentrations as low as nanomolar levels. A possible regulatory mechanism of phosphate uptake in marine Synechococcus spp. is discussed in relation to a wide distribution of this picophytoplankton in the ocean environment.
- リンク情報
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- DOI
- https://doi.org/10.1007/s002270050160
- CiNii Articles
- http://ci.nii.ac.jp/naid/10020011095
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1997XQ30000022&DestApp=WOS_CPL
- 共同研究・競争的資金等の研究課題
- 海洋性ラン藻Synechococcusの耐貧栄養機構の解明
- ID情報
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- DOI : 10.1007/s002270050160
- ISSN : 0025-3162
- CiNii Articles ID : 10020011095
- Web of Science ID : WOS:A1997XQ30000022