論文

国際誌
2020年12月

Sphingosine Kinase 1 is Associated With Immune Cell-Related Gene Expressions in Human Breast Cancer.

The Journal of surgical research
  • Junko Tsuchida
  • ,
  • Masayuki Nagahashi
  • ,
  • Masato Nakajima
  • ,
  • Eriko Katsuta
  • ,
  • Omar M Rashid
  • ,
  • Qianya Qi
  • ,
  • Li Yan
  • ,
  • Shujiro Okuda
  • ,
  • Kazuaki Takabe
  • ,
  • Toshifumi Wakai

256
開始ページ
645
終了ページ
656
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jss.2020.06.057

BACKGROUND: Although previous experiments have implicated sphingosine-1-phosphate (S1P) as a links between immune reactions and cancer progression, the exact mechanism of this interaction has not comprehensively studied in clinical human samples. This study sought to evaluate the S1P regulation by sphingosine kinase 1 (SPHK1), an S1P-producing enzyme, in the immunity/immuno-reactivity of clinical human breast cancer surgical specimens. METHODS: S1P levels were examined in tumor, peritumoral, and normal human breast samples using mass spectrometry. Genomics Data Commons data portal of The Cancer Genome Atlas cohort was used to assess the expression of S1P-related and immune-related genes. RESULTS: S1P levels were significantly higher in tumor samples compared to peritumoral (P < 0.05) or normal human breast samples (P < 0.001). SPHK1 gene expression was elevated in tumoral samples compared to normal breast samples (P < 0.01). Furthermore, the elevated expression of SPHK1 in breast cancer tissue was associated with an increased expression of the different kinds of immune-related genes, such as CD68, CD163, CD4, and FOXP3 (forkhead box P3), in HER2-negative breast cancer. Network analysis showed the central role of SPHK1 in the interaction of S1P signaling and expression of immune cell-related proteins. CONCLUSIONS: We demonstrated that S1P is mainly produced by tumor tissue, rather than peritumoral tissue, in breast cancer patients. Our data revealed the involvement of S1P signaling in the regulation of immune-related genes, suggesting the links between S1P and complicated immune-cancer interactions in breast cancer patients.

リンク情報
DOI
https://doi.org/10.1016/j.jss.2020.06.057
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32810665
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882641
ID情報
  • DOI : 10.1016/j.jss.2020.06.057
  • PubMed ID : 32810665
  • PubMed Central 記事ID : PMC7882641

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