2003年1月15日
Determination of urinary 12(S)-hydroxyeicosatetraenoic acid by liquid chromatography-tandem mass spectrometry with column-switching technique: sex difference in healthy volunteers and patients with diabetes mellitus.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
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- 巻
- 783
- 号
- 2
- 開始ページ
- 383
- 終了ページ
- 9
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
We developed a determination method for human urinary 12-hydroxyeicosatetraenoic acid (12-HETE) using LC-MS-MS. This method, which includes simple extraction and detection in the SRM mode, allows precise and accurate determination of 12-HETE. There was a significant sex difference in urinary 12-HETE levels. Chiral analysis of 12-HETE using LC-MS-MS with column-switching technique revealed that the major enantiomer was 12(S)-HETE. Furthermore, the urinary level in patients with diabetes mellitus (DM) was analyzed. The present in vivo findings indicate that there could be difference in production of 12(S)-HETE between genders and 12(S)-HETE may play a role in the pathogenesis of DM.
- リンク情報
- ID情報
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- ISSN : 1570-0232
- PubMed ID : 12482481