2009年10月
Ambient imaging mass spectrometry by electrospray ionization using solid needle as sampling probe.
Journal of mass spectrometry : JMS
- 巻
- 44
- 号
- 10
- 開始ページ
- 1469
- 終了ページ
- 77
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/jms.1632
- 出版者・発行元
- JOHN WILEY & SONS LTD
Although being an atmospheric pressure ion source, electrospray ionization (ESI) has rarely been used directly for ambient imaging mass spectrometry because the sample has to be introduced as liquid solution through the capillary. Instead of capillary, probe electrospray ionization (PESI), which has been developed recently, uses a solid needle as the sampling probe, as well as the electrospray emitter, and has been applied not only for liquid solutions but also for the direct sampling on wet samples. Biological tissues are composed of cells that contain 70-90% water, and when the surface is probed by the needle tip, the biological fluid adhering to the needle can be electrosprayed directly or assisted by additional solvent added onto the needle surface. Here, we demonstrate ambient imaging mass spectrometry of mouse brain section using PESI, incorporated with an auxiliary heated capillary sprayer. The solvent vapor generated from the sprayer condensed on the needle tip, re-dissolving the adhered sample, and at the same time, providing an indirect means for needle cleaning. The histological sections were prepared by fixation using paraformaldehyde, and the spatial analysis was automated by maintaining an equal sampling depth into the sample in addition to raster scan. Phospholipids and galactosylceramides were readily detected from the mouse brain section in the positive ion mode, and were mapped with 60 gm lateral resolution to form mass spectrometric images. Copyright (C) 2009 John Wiley & Sons, Ltd.
- リンク情報
- ID情報
-
- DOI : 10.1002/jms.1632
- ISSN : 1076-5174
- PubMed ID : 19685483
- Web of Science ID : WOS:000271722500007