2019年2月
Use of ionic liquid for X-ray micro-CT specimen preparation of imbibed seeds
MICROSCOPY
- 巻
- 68
- 号
- 1
- 開始ページ
- 92
- 終了ページ
- 97
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/jmicro/dfy130
- 出版者・発行元
- OXFORD UNIV PRESS
X-ray micro-CT is one of the most useful techniques to examine 3D cellular architecture inside dry seeds. However, the examination of imbibed seeds is difficult because immersion in water causes a decline in the image quality. Here, we examined the use of ionic liquids for specimen preparation of chemically fixed imbibed seeds of Arabidopsis. We found that treatment with high concentrations of ionic liquids after osmium tetroxide fixation helped not only to prevent the structural damage caused by seed shrinkage, but also to preserve the image quality. Under these conditions, the cellular architecture of seeds was also well maintained.
- リンク情報
- ID情報
-
- DOI : 10.1093/jmicro/dfy130
- ISSN : 2050-5698
- eISSN : 2050-5701
- Web of Science ID : WOS:000460633000010