論文

査読有り 国際誌
2020年5月

Plasmodium infection cure cycles induce modulation of conventional dendritic cells.

Microbiology and immunology
  • Ryosuke Adachi
  • ,
  • Takahiko Tamura

担当区分
責任著者
64
5
開始ページ
377
終了ページ
386
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/1348-0421.12783

Malaria is one of the most widespread human infectious diseases worldwide and a cause of mortality. It is difficult to induce immunological memory against the malarial parasite Plasmodium. The immunity to clinical malaria disease is acquired with multiple infection and treatment cycles, along with substantial reduction in parasite burden. However, the mechanism of the acquired immunity remains largely unclear. Conventional DCs (cDCs) play a pivotal role in orchestration of immune responses. The purpose of this study is to analyze the characterization of cDCs after the infection and cure treatment cycles. Mice were infected with the lethal rodent malarial parasite Plasmodium berghei ANKA, which was followed by cure treatment with the antimalarial drug pyrimethamine. This was then followed by a challenge with live parasites. The mice that went through infection cure cycles showed significant immune response, demonstrating robust immunological memory against malaria parasites. We investigated the cytokine production capacity of splenic cDCs in both naive and infection cure mice by stimulating purified splenic cDCs with LPS (TLR4 agonist) or CpG (TLR9 agonist). The capacity of cytokine production was found to be significantly decreased in infection cure mice. The suppression of cytokine production was sustained for a long term (6 months). Moreover, the surface expression of MHC Class II molecules was significantly lower in infection cure mice than in naive mice. These results suggest that Plasmodium infection and cure treatment resulted in strong immunological memory and modulation of full functionality of cDCs.

リンク情報
DOI
https://doi.org/10.1111/1348-0421.12783
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32096562
共同研究・競争的資金等の研究課題
マラリア原虫感染時における樹状細胞の発生分化プログラムに関する研究

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