Apr, 2015 - Mar, 2018
Development of next generation of baculovirus vectors with tissue specific gene transduction
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
- Grant number
- 15K07926
- Japan Grant Number (JGN)
- JP15K07926
- Grant amount
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- (Total)
- 4,810,000 Japanese Yen
- (Direct funding)
- 3,700,000 Japanese Yen
- (Indirect funding)
- 1,110,000 Japanese Yen
In this research, budded recombinant baculovirus vectors (BVs) have been developed because BVs have many advantages for tissue targeting gene delivery vectors.It is easily possible to display exogenous proteins on the surface of BV virion. CSP and TRAP, malaria sporozoite surface proteins have highly selective affinity to hepatocytes. BVs expressing CSP or TRAP molecule on the virion surface have been constructed and they were shown to greatly increase transduction efficacy to human hepatoma cell line (HepG2 et al.) and human primary hepatocytes (PXB cells). Furthermore, to overcome the vulnerability to serum complement system, fusion molecules of complement regulatory proteins were displayed to BV virion surfaces. These BVs were shown to greatly increase resistance against complement attack.
- Link information
- ID information
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- Grant number : 15K07926
- Japan Grant Number (JGN) : JP15K07926
List of results of the research project
Narrowing down
Papers
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BIOLOGICAL & PHARMACEUTICAL BULLETIN, 46(3) 404-411, Mar, 2023 Peer-reviewedLast authorCorresponding author
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Biological and Pharmaceutical Bulletin, 44(2), Feb, 2021 Peer-reviewedLast authorCorresponding author
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Biological & pharmaceutical bulletin, 41(10) 1600-1605, 2018 Peer-reviewedCorresponding author
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JOURNAL OF GENE MEDICINE, 18(4-6) 75-85, Apr, 2016 Peer-reviewedLead authorCorresponding author