Kazuhiro CHIKU

J-GLOBAL         Last updated: Jul 30, 2019 at 17:39
 
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Name
Kazuhiro CHIKU
Affiliation
Nippon Veterinary and Life Science University
Section
Faculty of Applied Life Science
Job title
Assistant Professor
Research funding number
30711618
ORCID ID
0000-0001-6857-8894

Research Areas

 
 

Academic & Professional Experience

 
Apr 2018
 - 
Today
Assistant Professor, Faculty of Applied Life Science, Nippon Veterinary and Life Science University
 
Oct 2014
 - 
Mar 2018
Research Associate, Faculty of Applied Life Science, Nippon Veterinary and Life Science University
 
Apr 2013
 - 
Sep 2014
Postdoctoral fellow, Faculty of agriculture, Niigata University
 
Apr 2012
 - 
Mar 2013
Postdoctoral fellow, Nanosystem Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
 
Apr 2011
 - 
Mar 2012
Postdoctoral fellow, National Food Research Institute (NFRI)
 

Published Papers

 
Mitsuru Yoshida, Mio Ichikawa, Itsuki Tomita, Kazuhiro Chiku, Mayumi Hachinohe, Shioka Hamamatsu and Hiroshi Okadome
Nippon Shokuhin Kagaku Kogaku Kaishi   66(2) 47-51   Feb 2019   [Refereed]
Mitsuru Yoshida, Marino Isamu, Kazuhiro Chiku
Food Safety   5(2) 54-60   Jun 2017   [Refereed]
Acrylamide has neurotoxicity, carcinogenicity, and genotoxicity in experimental animals and cellular systems. Fried potato is one of the major intake sources of acrylamide in food, and fried onion was reported to contain up to 100 ng/g level of ac...
Tomohiro Tsuda, Takanori Nihira, Kazuhiro Chiku, Erika Suzuki, Takatoshi Arakawa, Mamoru Nishimoto, Motomitsu Kitaoka, Hiroyuki Nakai, Shinya Fushinobu
FEBS Letters   589(24 Pt B) 3816-3821   Nov 2015   [Refereed]
Kazuhiro Chiku(Co-first), Takanori Nihira(Co-first), Erika Suzuki, Mamoru Nishimoto, Shinya Fushinobu, Motomitsu Kitaoka, Ken’ichi Ohtsubo, Hiroyuki Nakai
FEBS Letters   589(23) 3604-3610   Oct 2015   [Refereed]
Chiku Kazuhiro, Nihira Takanori, Suzuki Erika, Nishimoto Mamoru, Kitaoka Motomitsu, Nakai Hiroyuki, Ohtsubo Ken'ichi
Bulletin of applied glycoscience   5(2) 120-127   May 2015   [Refereed][Invited]
偏性嫌気性菌Bacteroides thetaiotaomicronおよび好熱性嫌気性菌Thermoanaerobacter sp. X-514から,糖質加水分解酵素ファミリー130に分類される3種の新規なβ-マンノシドホスホリラーゼ(BT_1033,Teth514_1788およびTeth514_1789)を見出した。BT_1033は,β-1,4-マンノシル-N-アセチルグルコサミンをリン酸存在下で可逆的に加リン酸分解する新規酵素であり,β-1,4-マンノシル-N-アセチルグルコサミンホス...
Kazuhiro Chiku, Takanori Nihira, Erika Suzuki, Mamoru Nishimoto, Motomitsu Kitaoka, Ken’ichi Ohtsubo, and Hiroyuki Nakai
PLoS One   9(12) e114882   Dec 2014   [Refereed]
We characterized Teth514_1788 and Teth514_1789, belonging to glycoside hydrolase family 130, from Thermoanaerobacter sp. X-514. These two enzymes catalyzed the synthesis of 1,2-β-oligomannan using β-1,2-mannobiose and d-mannose as the optimal acce...
Nihira Takanori, Miyajima Futaba, Chiku Kazuhiro, Kitaoka Motomitsu, Nakai Hiroyuki, Ohtsubo Ken'ichi
Bulletin of applied glycoscience   4(2) 147-153   May 2014   [Refereed][Invited]
嫌気性土壌細菌Clostridium phytofermentansから糖加水分解酵素ファミリー65に分類される4種のホスホリラーゼ(Cphy_1019,Cphy_1874,Cphy_3313およびCphy_3314)を見出だした。Cphy_1874およびCphy_3313は,α-1,3-グルコ二糖であるニゲロースをリン酸存在下で加リン酸分解する新規酵素ニゲロースホスホリラーゼであることが判明した。またCphy_1019は,逆反応においてラムノースのみをアクセプターとして利用する新規酵素3...
Takanori Nihira, Futaba Miyajima, Kazuhiro Chiku, Mamoru Nishimoto, Motomitsu Kitaoka, Ken’ichi Ohtsubo, Hiroyuki Nakai
Journal of Applied Glycoscience   61(3) 75-80   Apr 2014   [Refereed]
One-pot enzymatic production of nigerose was demonstrated from abundantly available sugar resources, including maltose, cellobiose, sucrose and starch. (i) 319 mM nigerose was generated from 500 mM maltose by the combined actions of maltose phosph...
Takanori Nihira, Yuka Saito, Kazuhiro Chiku, Motomitsu Kitaoka, Ken’ichi Ohtsubo, and Hiroyuki Nakai
FEBS Letters   587(21) 3382-3386   Nov 2013   [Refereed]
We discovered a potassium ion-dependent trehalose phosphorylase (Bsel_1207) belonging to glycoside hydrolase family 65 from halophilic Bacillus selenitireducens MLS10. Under high potassium ion concentrations, the recombinant Bsel_1207 produced in ...
Kazuhiro Chiku, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Tadashi Ishii, Mitsuru Yoshida, Fumiko Taguchi, Yuki Ichinose, and Hiroshi Ono
Carbohydrate research   375 100-104   May 2013   [Refereed]
Flagellin is a principal component of the flagellum filament. Previously, we reported that the flagellin of Pseudomonas syringae pv. tabaci 6605 (Pta6605) was glycosylated by oligosaccharides composed of two or three l-rhamnosyl (l-Rha) residues a...
Kazuhiro Chiku, Kazuhiko Tsunemi, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Tadashi Ishii, Mitsuru Yoshida, Fumiko Taguchi, Masako Iwaki, Yuki Ichinose, and Hiroshi Ono
Bioscience, Biotechnology, and Biochemistry   77(3) 505-510   Mar 2013   [Refereed]
D-rhamnose (D-Rha) residue is a major component of lipopolysaccharides (LPSs) in strains of the phytopathogen Pseudomonas syringae pathovar glycinea. To investigate the effects of a deficiency in GDP-D-rhamnose biosynthetic genes on LPS structure ...
Yuki Ichinose, Fumiko Taguchi, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Tatsuo Atsumi, Masako Iwaki, Hiromi Manabe, Mio Kumagai, Quan Thanh Nguyen, Chi Linh Nguyen, Yoshishige Inagaki, Hiroshi Ono, Kazuhiro Chiku, Tadashi Ishii, Mitsuru Yoshida
Journal of General Plant Pathology   79(5) 359-365   Jul 2013   [Refereed]
Glycosylation of flagellin is known to be involved in filament stabilization, motility, and virulence in Pseudomonas syringae. Here we investigated flagellin glycosylation in other phytopathogenic bacteria. Analyses of deduced amino acid sequences...
Taketoshi Iwata, Kazuhiro Chiku, Ken-ichi Amano, Masahiro Kusumoto, Mayumi Ohnishi-Kameyama, Hiroshi Ono, Masato Akiba
PLoS One   8(2) e56900   Feb 2013   [Refereed]
Campylobacter jejuni is the most common bacterium that causes diarrhea worldwide, and chickens are considered the main reservoir of this pathogen. This study investigated the effects of serial truncation of lipooligosaccharide (LOS), a major compo...
Takanori Nihira, Hiroyuki Nakai, Kazuhiro Chiku, and Motomitsu Kitaoka
Applied Microbiology and Biotechnology   93(4) 1513-1522   Feb 2012   [Refereed]
A novel phosphorylase from Clostridium phytofermentans belonging to the glycoside hydrolase family (GH) 65 (Cphy1874) was characterized. The recombinant Cphy1874 protein produced in Escherichia coli showed phosphorolytic activity on nigerose in th...
Kitaoka Motomitsu, Nishimoto Mamoru, Inoue Kousuke, Chiku Kazuhiro, Nakajima Masahiro
Bulletin of applied glycoscience   2(2) 136-141   May 2012   [Refereed][Invited]
ビフィズス菌の菌体内にガラクト-N-ビオース(GNB,Galβ1,3GalNAc)およびラクト-N-ビオースI(LNB,Galβ1,3GlcNAc)を選択的に代謝するGNB/LNB経路を発見した。GNB/LNB経路に含まれる酵素群およびそのホモログを用いた実用的なオリゴ糖調製法について報告する。スクロースとGlcNAcを基質として,β-1,3-ガラクトシル-N-アセチルヘキソサミンホスホリラーゼ(GLNBP)を含む四種類の酵素を同時に作用させることによりLNBを高収率にて調製した。LNB調...
Linh Chi Nguyen, Fumiko Taguchi, Quang Minh Tran, Kana Naito, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Hiroshi Ono, Mitsuru Yoshida, Kazuhiro Chiku, Tadashi Ishii, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi, and Yuki Ichinose
Molecular Plant Pathology   13(7) 764-774   Sep 2012   [Refereed]
Type IV pilin (PilA) is a major constituent of pilus and is required for bacterial biofilm formation, surface motility and virulence. It is known that mature PilA is produced by cleavage of the short leader sequence of the pilin precursor, followe...
Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Chi L. Nguyen, Fumiko Taguchi, Kazuhiro Chiku, Tadashi Ishii, Hiroshi Ono, Mitsuru Yoshida, and Yuki Ichinose
Genes   2(4) 788-803   Oct 2011   [Refereed]
Previously we revealed that flagellin proteins in Pseudomonas syringae pv. tabaci 6605 (Pta 6605) were glycosylated with a trisaccharide, modified viosamine (mVio)-rhamnose-rhamnose and that glycosylation was required for virulence. We further ide...
Ikuo Sato, Jun Yoshikawa, Akiko Furusawa, Kazuhiro Chiku, Seigo Amachi, Takaaki Fujii
Bioscience, Biotechnology, and Biochemistry   74(1) 75-81   Jan 2010   [Refereed]
Malic enzyme (ME) was purified as an electrophoretically homogenous protein from Rhodopseudomonas palustris No. 7. The molecular weight of ME was estimated to be 650 kDa and that of its subunit, 86 kDa. ME activity was remarkably enhanced by di- a...
Kazuhiro Chiku(corresponding author), Jun Uzawa, Hiroko Seki, Seigo Amachi, Takaaki Fujii, and Hirofumi Shinoyama
Bioscience, Biotechnology, and Biochemistry   72(9) 2285-2293   Sep 2008   [Refereed]
A culture filtrate of Bacillus sp. KT12 was used to prepare polyphenyl β-oligoxylosides from xylan and polyphenols in a one-step reaction. One oligoxyloside transfer enzyme was purified from multiple xylanolytic enzymes in the culture filtrate. N-...
Kazuhiro Chiku, Mamoru Nishimoto, Motomitsu Kitaoka
Carbohydrate Research   345(13) 1901-1908   Sep 2010   [Refereed]
β-d-Galactopyranosyl-(1→3)-2-acetamido-2-deoxy-d-glucose (LNB) and β-d-galactopyranosyl-(1→3)-2-acetamido-2-deoxy-d-galactose (GNB) decompose rapidly upon heating into d-galactose and mono-dehydrated derivatives of the corresponding 2-acetamido-2-...

Misc

 
Evaluation of thermal decomposition behavior of various oligosaccharides and amino sugars under neutral pH conditions
Kazuhiro CHIKU
Agricultural Biotechnology   1(4) 94-99   Apr 2017   [Invited]
Masanori Saito, Hirofumi Shinoyama, Akihiro Saito, Kazuhiro Chiku, Akikazu Ando
HortResearch   (65) 75-80   Mar 2011   [Refereed]
Pestalotiopsis spp. strains were isolated from leaves of 17 out of 23 plant species. Some of the Pestalotiopsisspp. isolates showed catechol-xylosylation activity. A catechol-xylosylating enzyme was purified from the culturefiltrate of an isolated...
Masanori Saito, Hirofumi Shinoyama, Akihiro Saito, Kazuhiro Chiku, Akikazu Ando
HortResearch   (64) 35-41   Mar 2010   [Refereed]
市販されている各種糸状菌由来の糖質分解酵素剤に注目し、加水分解活性が弱く配糖化活性が強い酵素の有無を調べ、その精製を試みた。その結果、Trichoderma sp.由来の市販キシラン分解酵素剤に、キシランのみを基質とした場合、その加水分解活性がないにも関わらず、キシランとカテコール共存下ではキシランを分解しカテコールを配糖化する酵素が認められた。本酵素は分子量約73,000の単量体で機能するものであり、20残基のN末端アミノ酸配列は既知のキシラナーゼと相同性が認められなかった。これらの諸性...

Conference Activities & Talks

 
Application of light element stable isotopes to discrimination of geographical origin of rice
Arisa Hosonuma, Misaki Ban, Shiho Kuwahara, Mami Sawada, Ayana Tabata, Kazuhiro Chiku, Mitsuru Yoshida, Yaeko Suzuki
The Japanese Society of Appilied Glycoscience   3 Sep 2017   The Japanese Society of Applied Glycoscience
Epimerization of kojibiose and sophorose by heat treatment under neutral conditions
Kazuhiro CHIKU, Arisa Hosonuma, Hiroshi Ono, Motomitsu Kitaoka, Mitsuru Yoshida
The Japanese Society of Appilied Glycoscience   3 Sep 2017   The Japanese Society of Applied Glycoscience
Feeding preference of Japanese serow (Capricornis crispus) in cabbages
Takuya KASAI, Tomohiko ENDO, Yoshiyuki OZEKI, Kazuhiro CHIKU, Asako NARAI-KANAYAMA, Keitaro SUZUKI, Takahisa HOSODA, Hiroshi KAJIGAYA, Mitsuru YOSHIDA
22 Nov 2015   
Recent Development of Phosphorylase Possessing Large Potential for Oligosaccharide Synthesis
Kazuhiro CHIKU
10th Carbohydrate Bioengineering Meeting Satellite Workshop   Apr 2013   
Heat decomposition of Galβ1,3HexNAc under neutral condition
K. Chiku, M. Nishimoto, M. Kitaoka
25th International Carbohydrate Symposium (ICS 2010)   2010   

Research Grants & Projects

 
Characterization of milk oligosaccharide
Project Year: 2009