Nov, 2015
An inverting beta-1,2-mannosidase belonging to glycoside hydrolase family 130 from Dyadobacter fermentans
FEBS LETTERS
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- Volume
- 589
- Number
- 23
- First page
- 3604
- Last page
- 3610
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.febslet.2015.10.008
- Publisher
- ELSEVIER SCIENCE BV
The glycoside hydrolase family (GH) 130 is composed of inverting phosphorylases that catalyze reversible phosphorolysis of beta-D-mannosides. Here we report a glycoside hydrolase as a new member of GH130. Dfer_3176 from Dyadobacter fermentans showed no synthetic activity using alpha-D-mannose 1-phosphate but it released alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration, indicating that Dfer_3176 is a beta-1,2-mannosidase. Mutational analysis indicated that two glutamic acid residues are critical for the hydrolysis of beta-1,2-mannotriose. The two residues are not conserved among GH130 phosphorylases and are predicted to assist the nucleophilic attack of a water molecule in the hydrolysis of the beta-D-mannosidic bond. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
- Link information
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- DOI
- https://doi.org/10.1016/j.febslet.2015.10.008
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/26476324
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000367232100014&DestApp=WOS_CPL
- Research Projects
- Elucidation of the Novel Phosphorylase-Dependent Metabolisms of Glycosonjugates and Lipopolysaccharides
- URL
- http://www.febsletters.org/article/S0014-5793(15)00889-3/abstract
- ID information
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- DOI : 10.1016/j.febslet.2015.10.008
- ISSN : 0014-5793
- eISSN : 1873-3468
- Pubmed ID : 26476324
- Web of Science ID : WOS:000367232100014