Naoki Wada

Profile Information

Affiliation: Designated Assistant Professor, Graduate School of Technology, Industrial and Social Sciences, Tokushima University

Degree: 修士（工学）(大阪大学)
博士(工学)(大阪大学)

J-GLOBAL ID: 200901049385824987
researchmap Member ID: 6000020075

Research Interests

Research Areas

Research History

Oct, 2017 - Present

特任助教, 社会産業理工学研究部生物資源産業学域, 徳島大学大学院
Aug, 2017 - Sep, 2017

学術研究員, 社会産業理工学研究部生物資源産業学域, 徳島大学大学院
Apr, 2014 - Jul, 2017

Designated Assistant Professor, Osaka University
Apr, 2011 - Mar, 2014

JSPS Reserch Fellow PD, Tottori University
Apr, 2010 - Mar, 2011

Postdoc, Osaka University
Apr, 2007 - Mar, 2010

JSPS Research Fellow DC1, Osaka University
2007 - 2010

Special researcher of Japan Society for the Promotion of Science

Education

Apr, 2007 - Mar, 2010

生命先端工学専攻博士後期課程, 工学研究科, 大阪大学大学院
Apr, 2005 - Mar, 2007

生命先端工学専攻博士前期課程, 工学研究科, 大阪大学大学院
Apr, 2001 - Mar, 2005

Department of Applied Science, School of Engineering, Osaka University

Committee Memberships

Nov, 2016 - Present

評議員, 一般財団法人染色体学会

Awards

May, 2015

Best Poster Award, A hybrid cell line between human and plant, The 5th Asian Chromosome Colloquium

Wada Naoki, Yasuhiro Kazuki, Kiichi Fukui, MItuso Oshimura

Papers

Adenine sulfate and glutamine enhanced shoots multiplication in Jatropha curcas L. a potent biofuel plant

Mohammed A. Ismail, Tsuchimoto Suguru, Sakai Hiroe, Wada Naoki, Fukui Kiichi

African Journal of Biotechnology, Vol.20(No.9) 383-388, Sep, 2021 Peer-reviewed
Genome editing in mammalian cells using the CRISPR type I-D nuclease

Osakabe Keishi, Wada Naoki, Emi Murakami, Naoyuki Miyashita, Osakabe Yuriko

Nucleic Acids Research, Jul 2, 2021 Peer-reviewedLead author
Epigenetic Distribution of Recombinant Plant Chromosome Fragments in a HumanArabidopsis Hybrid Cell Line

Liaw YengMun, Liu Yikun, Teo CheeHow, Cápal Petr, Wada Naoki, Fukui Kiichi, Doležel Jaroslav, Ohmido Nobuko.

International Journal of Molecular Sciences, Vol.22(No.11) 5426-5426, May 21, 2021 Peer-reviewed
Molecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines

Liu Yikun, Liaw Mun Yeng, Teo How Chee, Cápal Petr, Wada Naoki, Fukui Kiichi, Doležel Jaroslav, Ohmido Nobuko

Scientific Reports, 11(1) 7160-7160, Mar 30, 2021 Peer-reviewed
Effects of the sliaa9 Mutation on Shoot Elongation Growth of Tomato Cultivars

Abe-Hara Chihiro, Yamada Kohji, Wada Naoki, Ueta Risa, Hashimoto Ryosuke, Osakabe Keishi, Osakabe Yuriko

Frontiers in Plant Science, 12 627832-627832, Mar 20, 2021 Peer-reviewed
Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering

Naoki Wada, Risa Ueta, Yuriko Osakabe, Keishi Osakabe

BMC Plant Biology, 20(1), Dec, 2020 Lead author
Genome editing in plants using CRISPR type I-D nuclease.

Osakabe Keishi, Wada Naoki, Tomoko Miyaji, Emi Murakami, Kazuya Marui, Risa Ueta, Ryosuke Hashimoto, Chihiro Abe-Hara, Bihe Kong, Kentaro Yano, Osakabe Yuriko

Communications Biology, Vol.3(1) 648-648, Nov 6, 2020 Peer-reviewed
Association Study of Seed-Yield Related Traits for Jatropha curcas L. in Mexico

Tropical Agriculture and Development, 62(2) 68-77, Oct, 2018 Peer-reviewed
Maintenance and Function of a Plant Chromosome in Human Cells

Naoki Wada, Yasuhiro Kazuki, Kanako Kazuki, Toshiaki Inoue, Kiichi Fukui, Mitsuo Oshimura

ACS SYNTHETIC BIOLOGY, 6(2) 301-310, Feb, 2017 Peer-reviewedLead author
Genetic Tracing of Jatropha curcas L. from Its Mesoamerican Origin to the World.

Haiyan Li, Suguru Tsuchimoto, Kyuya Harada, Masanori Yamasaki, Hiroe Sakai, Naoki Wada, Atefeh Alipour, Tomohiro Sasai, Atsushi Tsunekawa, Hisashi Tsujimoto, Takayuki Ando, Hisashi Tomemori, Shusei Sato, Hideki Hirakawa, Victor P Quintero, Alfredo Zamarripa, Primitivo Santos, Adel Hegazy, Abdalla M Ali, Kiichi Fukui

Frontiers in plant science, 8 1539-1539, 2017 Peer-reviewed
High-frequency Shoots Regeneration of an Oil Crop, Simmondsia chinensis (Link) Schneider Using Axillary Buds.

Mohammed I. A, Tsuchimoto S, Sakai H, Wada N, Fukui K

Tropical Agriculture and Development., 2017 Peer-reviewed
Agrobacterium-Mediated Transformation of Jojoba [Simmondsia chinensis (Link) Schneider]

Mohammed I. A, Tsuchimoto S, Sakai H, Wada N, Fukui K

Tropical Agriculture and Development, 2017 Peer-reviewed
Localization of transgene-derived friabilins in rice endosperm cells

Misa Fujiwara, Go Suzuki, Daiki Kudo, Haruna Oba, Yukio Wada, Hideo Wada, Naoki Wada, Sadequr Rahman, Kiichi Fukui, Yasuhiko Mukai

PLANT BIOTECHNOLOGY, 31(1) 67-70, 2014 Peer-reviewed
Development of transgenic plants in jatropha with drought tolerance

Suguru Tsuchimoto, Joyce Cartagena, Naruemon Khemkladngoen, Suthitar Singkaravanit, Tsutomu Kohinata, Naoki Wada, Hiroe Sakai, Yoshihiko Morishita, Hideyuki Suzuki, Daisuke Shibata, Kiichi Fukui

PLANT BIOTECHNOLOGY, 29(2) 137-143, 2012 Peer-reviewed
Sequence Analysis of the Genome of an Oil-Bearing Tree, Jatropha curcas L.

Shusei Sato, Hideki Hirakawa, Sachiko Isobe, Elgo Fukai, Akiko Watanabe, Midori Kato, Kumiko Kawashima, Chiharu Minami, Akiko Muraki, Naomi Nakazaki, Chika Takahashi, Shinobu Nakayama, Yoshie Kishida, Mitsuyo Kohara, Manabu Yamada, Hisano Tsuruoka, Shigemi Sasamoto, Satoshi Tabata, Tomoyuki Aizu, Atsushi Toyoda, Tadasu Shin-i, Yohei Minakuchi, Yuji Kohara, Asao Fujiyama, Suguru Tsuchimoto, Shin'ichiro Kajiyama, Eri Makigano, Nobuko Ohmido, Nakako Shibagaki, Joyce A. Cartagena, Naoki Wada, Tsutomu Kohinata, Alipour Atefeh, Shota Yuasa, Sachihiro Matsunaga, Kiichi Fukui

DNA RESEARCH, 18(1) 65-76, Feb, 2011
The effects of puroindoline b on the ultrastructure of endosperm cells and physicochemical properties of transgenic rice plant

Naoki Wada, Shin&apos, ichiro Kajiyama, Joyce A. Cartagena, Linyen Lin, Yukio Akiyama, Motoyasu Otani, Go Suzuki, Yasuhiko Mukai, Noriaki Aoki, Kiichi Fukui

JOURNAL OF CEREAL SCIENCE, 51(2) 182-188, Mar, 2010 Lead author
Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes

Naoki Wada, Shin&apos, ichiro Kajiyama, Yukio Akiyama, Shigeki Kawakami, Daisuke No, Susumu Uchiyama, Motoyasu Otani, Takiko Shimada, Naoko Nose, Go Suzuki, Yasuhiko Mukai, Kiichi Fukui

PLANT CELL REPORTS, 28(5) 759-768, May, 2009 Lead author

Misc.

Expanding the plant genome editing toolbox with recently developed CRISPR-Cas systems

Wada Naoki, Osakabe Keishi, Osakabe Yuriko

Plant Physiology, Jan, 2022
新しいゲノム編集ツールTiDシステムの開発

和田直樹, 村上愛美, 宮下尚之, 刑部祐里子, 刑部敬史

日本分子生物学会年会プログラム・要旨集(Web), 43rd, 2020
細胞融合でヒトと植物の垣根を超えろ

Someone, (38), 2017 Invited
ヒトと植物細胞の細胞融合：16億年の時を超えた再会

和田直樹

現代化学, 41-43, 2017
不毛な大地で油を作る植物たち

和田直樹

公益社団法人日本生物工学会誌, 93(3) 156, 2015

Books and Other Publications

ゲノム編集食品 : 農林水産分野への応用と持続的社会の実現田部井豊監修

(担当共著) 和田直樹, 刑部祐里子, 刑部敬史, 第2章DNA編集技術, CRISPR, Casによるゲノム編集技術, 総論

エヌ・ティー・エス, Feb, 2021 (ISBN: 9784860437169)
最新のゲノム編集技術と用途展開山本卓監修

(担当共著) 和田直樹、刑部祐里子、刑部敬史、第9章高等動植物に利用可能な新規ゲノム編集ツールの開発

シーエムシー出版, Feb, 2021 (ISBN: 9784781315867)
第2章DNA編集技術 1. CRISPR/Casによるゲノム編集技術-総論

Osakabe Keishi, Wada Naoki

株式会社エヌ・ティー・エス, 2021
第9章高等動植物に利用可能な新規ゲノム編集ツールの開発

Wada Naoki, Osakabe Yuriko, Osakabe Keishi

株式会社シーエムシー出版, 2021
Synthetic Biology: Methods and Protocols, Editor: Braman, Jeffy Carl

Wada N, Kazuki Y, Kazuki K, Inoue T, Fukui K, Oshimura M, Production of Human Cell line with a Plant Chromosome (Role: Contributor)

Humana Press, 2018 (ISBN: 9781493977956)
Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes, with special reference to bead-production methodology.

Naruemon Khemkladngoen, Wada Naoki, Tsuchimoro Suguru, Cartagene A Joyce, Kajiyama Shin'ichiro, Fukui Kiichi

InTech, USA, 2012
Bioactive beads-mediated transformation of plants with large DNA fragments.

Wada Naoki, Khemkladngoen Naruemon, Cartagena A Joyce, Fukui Kiichi

Humana Press, 2012
Transgenic Plants-Advances and Limitations, Editor: Yelda Ozden

Wada N, Khemkladngoen N, Cartagena J.A, Fukui K., Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes with special reference to bead-production methodology (Role: Contributor)

InTech, USA, 2012 (ISBN: 9789535101819)
Transgenic Plants: Methods in Molecular Biology. Editor: Dunwell J, Wetten A

Wada N, Khemkladngoen N, Cartagena J.A, Fukui K., Bioactive beads-mediated transformation of, plants with large DNA fragments (Role: Contributor)

InTech, 2012
Plant Transformation Technologies. Editor: C. Neal Stewart Jr., Alisher Touraev, Vitaly Citovsky, and Tzvi Tzfira

Wada N, Kajiyama S, Khemkladngoen N, Fukui K., A novel gene delivery system in plants with calcium alginate micro-beads

Wiley-Blackwell Publishers, 2011

Presentations

新規ゲノム編集ツールTiDを用いたヒト細胞でのゲノム編集

Wada Naoki, 村上愛美, 丸井和也, 宮下尚之, Osakabe Yuriko, Osakabe Keishi

第73回日本生物工学会大会, Oct 28, 2021
植物染色体は動物細胞の中でどのように適応していくのか?

近江戸伸子, Yen Mun Liaw, Yukun Liu, Chee How Tel, Petr Capal, Wada Naoki, 福井希一, Jaroslav Doležel

一般財団法人染色体学会第72回(2021年度)年会, Sep 16, 2021
新規ゲノム編集ツールTiDシステムを用いた植物のゲノム編集

Wada Naoki, 宮地朋子, 村上愛美, 丸井和也, 上田梨紗, 橋本諒典, 阿部-原千尋, Bihe Kong, 矢野健太郎, Osakabe Yuriko, Osakabe Keishi

第62回日本植物生理学会年会, Mar 15, 2021
Development and characterization of a novel genome editing tool, the TiD system

Wada Naoki, Emi Murakami, Kazuya Marui, Ryosuke Hashimoto, Yuriko Osakabe, Keishi Osakabe

Keystone symposia on Molecular and Cellular Biology Plant Genome Engineering: From lab to Field, Mar 9, 2021
新しいゲノム編集ツールTiDシステムの開発

Wada Naoki, 村上愛美, 宮下尚之, 刑部祐里子, 刑部敬史

第43回日本分子生物学会年会, Dec 2, 2020
Genetics and Epigenetics of Plant Chromosomes in a Human-plant hybrid cell line

Liaw, YengMun, Liu, Yikun, Cápal, Petr, Doležel, Jaroslav, Teo, CheeHow, Wada Naoki, Fukui, Kiichi, Ohmido, Nobuko

Asia Pacific Chromosome Colloquium, Nov 25, 2020
in planta-regeneration法におけるdCas9-転写活性化ベクターを用いた遺伝子発現制御システムの開発

Risa Ueta, Nozomu Kira, Chihiro Hara, Tomoko Miyaji, Wada Naoki, Osakabe Yuriko, Osakabe Keishi

第61回日本植物生理学会年会, Mar 19, 2020
DEVELOPMENT OF A NOVEL GENOME EDITING TOOL, TID SYSTEM, FOR MAMMALIAN GENOME ENGINEERING

Wada Naoki, Emi Murakami, Ryosuke Hashimoto, Osakabe Yuriko, Osakabe Keishi

Frontiers in Genome Engineering 2019, Nov 26, 2019
Genome editing in plants by using a novel genome editing tool TiD

Osakabe Keishi, Wada Naoki, Kazuya Marui, Emi Murakami, Risa Ueta, Ryosuke Hashimoto, Chihiro Hara, Tomoko Miyaji, Osakabe Yuriko

Frontiers in Genome Engineering 2019, Nov 26, 2019
Reconstruction of Arabidopsis chromosome in human-plant hybrid cell

YiKun Liu, Yeng Mun Liaw, Petr Cápal, Jaroslav Dolezel, Chee How Teo, Wada Naoki, Kiichi Fukui, Nobuko Ohmido

染色体学会第70回年会, Sep 23, 2019
CRISPR/dCas9を利用した植物遺伝子発現制御システムの開発

Risa Ueta, Nozomu Kira, Rika Yoshioka, Tomoko Miyaji, Wada Naoki, Osakabe Yuriko, Osakabe Keishi

第37回日本植物細胞分子生物学会, Sep 8, 2019
Development of a highly sensitive guide RNA evaluation system using Nano Luciferase

Wada Naoki, Emi Murakami, Osakabe Yuriko, Osakabe Keishi

The 12th International Symposium Exploring the Global Sustainability, Kindai University, 201985, Aug 5, 2019
Nano Luciferaseを用いた高感度ガイドRNA評価システムの開発

Wada Naoki, 村上愛美, Osakabe Yuriko, Osakabe Keishi

日本ゲノム編集学会第4回大会, Jun 4, 2019
Control of plant gene expression by using CRISPR/dCas9 system

Ueta Risa, 宮地朋子, Wada Naoki, Osakabe Yuriko, Osakabe Keishi

第60回日本植物生理学会年会, Mar 13, 2019
Construction of hybrid cells between algae and human cultured cells toward creation of planimal cells

Sachihiro Matsunaga, Sumire Ishiyama, Wada Naoki, Tomoko Matsunaga

第30回日本植物形態学会, Sep 13, 2018
植物/ヒト雑種細胞における植物染色体の挙動と遺伝子発現

Wada Naoki, 香月康宏, 香月加奈子, 井上敏昭, Osakabe Keishi, 福井希一, 押村光雄

第59回日本植物生理学会, Mar 30, 2018
Maintenance and function of a plant chromosome in human cells

Wada Naoki

The 11th International Symposium Exploring the Global Sustainability, Mar 20, 2018
ヒトと植物細胞の部分的な融合細胞株の樹立 (招待講演)

Wada Naoki

第38回日本動物細胞工学会シンポジウム, Mar 7, 2018 Invited
ヒトと植物細胞の部分的な融合細胞株の樹立—進化を超えた染色体機能保存性の解明を目指してー (招待講演)

Wada Naoki

第5回細胞凝集研究会, Nov 17, 2017 Invited
Developing industrial oil-seed trees for drylands

Tsuchimoto S, Sakai H, Wada N, Fukui K

The 10th International symposium “Exploring the global sustainability -Advances in plant biotechnology for agriculture in semi-arid land”, Mar 15, 2017

Teaching Experience

細胞動態学 (大阪大学大学院)

Professional Memberships

Research Projects

乾燥地に適した産業用油糧作物の開発

鳥取大学乾燥地研究センター共同研究・重点研究, 鳥取大学乾燥地研究センター, Apr, 2016 - Mar, 2017

土本卓
国産ホホバオイル含有皮膚保湿剤の試作研究

平成27年度マッチングプランナープログラム「探索試験」, Oct, 2015 - Mar, 2017

土本卓
新規の植物育種のための植物人工染色体ベクター作出方法の確立

独立行政法人日本学術振興会特別研究員制度PD, 独立行政法人日本学術振興会, Apr, 2011 - Mar, 2014

和田直樹
新育種法のためのアポミクシス遺伝子座解析とその導入方法の開発

独立行政法人日本学術振興会特別研究員DC1, 独立行政法人日本学術振興会, Apr, 2007 - Mar, 2010

和田直樹
Genetic analysis and genetic modification of biofuel plant Jatropha curcas
Transformation of rice with large DNA fragments containing Aegilops tauschii hardness genes using bioactive beads method

Industrial Property Rights

特許6030281 ヤトロファ由来のPPATをコードするポリヌクレオチド及びその利用

福井希一, ジョイスカルタヘナ, 和田直樹, 小日向務, 湯塩泰久, 池口直樹, 田畑哲之

Profile Information

Research Interests

Research Areas

Research History

Education

Committee Memberships

Awards

Papers

Misc.

Books and Other Publications

Presentations

Teaching Experience

Professional Memberships

Research Projects

Industrial Property Rights

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