論文

査読有り
2006年2月

Decorin binds myostatin and modulates its activity to muscle cells

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • T Miura
  • ,
  • Y Kishioka
  • ,
  • J Wakamatsu
  • ,
  • A Hattori
  • ,
  • A Hennebry
  • ,
  • CJ Berry
  • ,
  • M Sharma
  • ,
  • R Kambadur
  • ,
  • T Nishimura

340
2
開始ページ
675
終了ページ
680
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2005.12.060
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Myostatin, a member of TGF-beta superfamily of growth factors, acts as a negative regulator of skeletal muscle mass. The mechanism whereby myostatin controls the proliferation and differentiation of myogenic cells is mostly clarified. However, the regulation of myostatin activity to myogenic cells after its secretion in the extracellular matrix (ECM) is still unknown. Decorin, a small leucine-rich proteoglycan, binds TGF-beta and regulates its activity in the ECM. Thus, we hypothesized that decorin could also bind to myostatin and participate in modulation of its activity to myogenic cells. In order to test the hypothesis, we investigated the interaction between myostatin and decorin by surface plasmon assay. Decorin interacted with mature myostatin in the presence of concentrations of Zn2+ greater than 10 mu M, but not in the absence of Zn2+. Kinetic analysis with a 1:1 binding model resulted in dissociation constants (K-D) of 2.02 x 10(-8) M and 9.36 x 10(-9) M for decorin and the core protein of decorin, respectively. Removal of the glycosaminoglycan chain by chondroitinase ABC digestion did not affect binding, suggesting that decorin could bind to myostatin with its core protein. Furthermore, we demonstrated that immobilized decorin could rescue the inhibitory effect of myostatin on myoblast proliferation in vitro. These results suggest that decorin could trap myostatin and modulate its activity to myogenic cells in the ECM. (c) 2005 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 119

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2005.12.060
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16380093
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000234731800046&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbrc.2005.12.060
  • ISSN : 0006-291X
  • PubMed ID : 16380093
  • Web of Science ID : WOS:000234731800046

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