2012年4月
Separation of a milk acid phosphatase from a purified lactoferrin fraction and identification as a member of the mammalian purple acid phosphatase family
INTERNATIONAL DAIRY JOURNAL
- ,
- ,
- ,
- 巻
- 23
- 号
- 2
- 開始ページ
- 86
- 終了ページ
- 90
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.idairyj.2011.10.003
- 出版者・発行元
- ELSEVIER SCI LTD
Active acid phosphatase (AcP), 37 kDa, was completely separated from the purified lactoferrin fraction of bovine milk (LF-rich fraction). The N-terminal amino acid sequence of the 37 kDa molecule had 84% homology with bovine uteroferrin (Uf)-like protein. The 37 kDa molecule has an optimum pH range of 4.5-5.0 and an optimum temperature of 60 degrees C. The AcP activity of the iron removal 37 kDa molecule (iron-depleted LF-rich fraction) was approximately half that of the iron-containing sample (LF-rich fraction). The activity increased in a time-dependent manner on tryptic digestion. These profiles correspond to the mammalian purple acid phosphatase (PAP) family (Uf, Type-5 and tartrate-resistant acid phosphatase: EC3.1.3.2). It seems reasonable to propose that the active molecule in the LF-rich fraction is an undocumented bovine PAP-family protein. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.idairyj.2011.10.003
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000299814000003&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84856281709&origin=inward
- ID情報
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- DOI : 10.1016/j.idairyj.2011.10.003
- ISSN : 0958-6946
- SCOPUS ID : 84856281709
- Web of Science ID : WOS:000299814000003