2016年12月
Detoxification of hydroxylated polychlorobiphenyls by Sphingomonas sp strain N-9 isolated from forest soil
CHEMOSPHERE
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- 巻
- 165
- 号
- 開始ページ
- 173
- 終了ページ
- 182
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.chemosphere.2016.08.127
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
To examine the biodegradation of hydroxylated polychlorobiphenyls (OH-PCBs), we isolated Sphingomonas sp. strain N-9 from forest soil using mineral salt medium containing 4-hydroxy-3-chlorobiphenyl (4OH-3CB) at the concentration of 10 mg/L. Following incubation with strain N-9, the concentration of 4OH-3CB decreased in inverse proportion to strain N-9 proliferation, and it was converted to 3-chloro-4-hydroxybenzoic acid (4OH-3CBA) after 1 day. We observed that strain N-9 efficiently degraded lowly chlorinated OH-PCBs (1-4 Cl), while highly chlorinated OH-PCBs (5-6 Cl) were less efficiently transformed. Additionally, strain N-9 degraded PCBs and OH-PCBs with similar efficiencies, and the efficiency of OH-PCB degradation was dependent upon the positional relationships between OH-PCB hydroxyl groups and chlorinated rings. OH-PCB biodegradation may result in highly toxic products, therefore, we evaluated the cytotoxicity of two OH-PCBs [4OH-3CB and 4-hydroxy-3,5-dichlorobiphenyl (4OH-3,5CB)] and their metabolites [4OH-3CBA and 3,5-chloro-4-hydroxybenzoic acid (4OH-3,5CBA)] using PC12 rat pheochromocytoma cells. Our results revealed that both OH-PCBs induced cell membrane damage and caused neuron-like elongations in a dose-dependent manner, while similar results were not observed for their metabolites. These results indicated that strain N-9 can convert OH-PCBs into chlorohydroxybenzoic acids having lower toxicity. (C) 2016 Published by Elsevier Ltd.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.chemosphere.2016.08.127
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/27649311
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000386402200021&DestApp=WOS_CPL
- URL
- http://orcid.org/0000-0002-2838-569X
- ID情報
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- DOI : 10.1016/j.chemosphere.2016.08.127
- ISSN : 0045-6535
- eISSN : 1879-1298
- ORCIDのPut Code : 45901231
- PubMed ID : 27649311
- Web of Science ID : WOS:000386402200021