2013年8月
Identification of the major capsid gene (g23) of T4-type bacteriophages that assimilate substrates from root cap cells under aerobic and anaerobic soil conditions using a DNA-SIP approach
Soil Biology and Biochemistry
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- 巻
- 63
- 号
- 開始ページ
- 97-105
- 終了ページ
- 105
- 記述言語
- 英語
- 掲載種別
- 速報,短報,研究ノート等(学術雑誌)
- DOI
- 10.1016/j.soilbio.2013.03.026
As the most abundant biological entities, viruses are increasingly recognised as a major driving force of global biogeochemical nutrient cycles. This study demonstrated that T4-type bacteriophages drive the microbial loop of carbon from root cap cells in rice rhizospheres based on an analysis of the major capsid gene (g23). Rice callus cells were used as a model for root cap cells, and 13C-labelled callus cells were allowed to decompose. DNA extracted from the soils after incubation under aerobic and anaerobic soil conditions was subjected to PCR-DGGE after density gradient centrifugation. Although 13C-labelled g23 fragments were not detected in soil incubated under anaerobic conditions, many 13C-labelled g23 fragments belonging to Paddy Groups V, VIII and IX were obtained from the soil incubated under aerobic conditions. Some g23 fragments were detected throughout the incubation period, and others were obtained only during the early or late incubation period. This study demonstrated that the level of infection of soil bacteria by T4-type phages was very different between aerobic and anaerobic soil conditions. Based on the results of this study, the roles of phages in the decomposition of the root cap cells of rice plants in rice fields are discussed. © 2013 Elsevier Ltd.
- ID情報
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- DOI : 10.1016/j.soilbio.2013.03.026
- ISSN : 0038-0717
- SCOPUS ID : 84876727714