2010年
Expression of the stress-related genes for glutathione S-transferase and ascorbate peroxidase in the most-glycinin-deficient soybean cultivar Tousan205 during seed maturation
Bioscience, Biotechnology and Biochemistry
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- 巻
- 74
- 号
- 9
- 開始ページ
- 1976
- 終了ページ
- 1979
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.100479
Global analysis of gene expression profiles in most-glycinin-deficient cultivar Tousan205, was performed by DNA microarray analysis. It was confirmed that Tousan205 lacks mRNA expression of three glycinin subunit precursor genes, Gl (AlaBlx), G2 (A2Bla), and G5 (A3B4), and lacks G4 (A5A4B3) protein. Most glycinin subunits were deficient in mature seeds of Tousan205. We compared the gene expression of Tousan205 with those of parent cultivar, Tamahomare, which was used for crossbreeding of Tousan205. As a result, Tousan205 exhibited higher expression of some seed maturation proteins, and stress-related genes such as glutathione S-transferase and ascorbate peroxidase. This result indicates the possibility that the decrease of main storage protein, glycinin causes stress in soybean.
- ID情報
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- DOI : 10.1271/bbb.100479
- ISSN : 0916-8451
- ISSN : 1347-6947
- PubMed ID : 20834138
- SCOPUS ID : 77957334138