2015年8月24日
Preparation and characterization of silver nanoparticles-reduced graphene oxide on ITO for immunosensing platform
Sensors and Actuators, B: Chemical
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- 巻
- 221
- 号
- 開始ページ
- 1423
- 終了ページ
- 1432
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.snb.2015.06.156
- 出版者・発行元
- ELSEVIER SCIENCE SA
We here report the in-situ electrochemical deposition of silver nanoparticles (AgNPs) and a reduced graphene oxide (rGO) nanocomposite on the surface of an indium tin oxide (ITO) electrode. The nanocomposite modified ITO was used as a basic platform for the construction of a novel electrochemical biosensor for the detection of hydrogen peroxide (H<inf>2</inf>O<inf>2</inf>); where horseradish peroxidase (HRP) tagged antibodies acted as recognition elements for carcinoembryonic antigen (CEA). The AgNPs-rGO/ITO modified electrode remarkably outperformed a bare electrode because of its enhanced surface area and electrocatalytic activity, resulting in a notably amplified electrical signal for the detection of H<inf>2</inf>O<inf>2</inf>. Through a cyclic voltammetric (CV) technique, the detection limit of the sandwich type immunoassay configuration was found to be 214 μM, with a linear range of 25-500 μM, as compared to the non-sandwich system, which was 120 μM with the same linear range. In contrast, the current-time response of the resulting sandwich-type immunoassay configuration showed a wider linear response to H<inf>2</inf>O<inf>2</inf> in the range of 25-1450 μM, with a detection limit of 5.3 μM, while the non-sandwich system exhibited a linear range of 25-1355 μM (R2 = 0.9992), with a detection limit of 10 μM. Therefore, the current-time response provided a more sensitive means of detecting H<inf>2</inf>O<inf>2.</inf> The resulting immunosensor also exhibited outstanding stability and reproducibility, as well as selectivity toward H<inf>2</inf>O<inf>2</inf> in the presence of several interferences.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.snb.2015.06.156
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000362918100180&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84939817236&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84939817236&origin=inward
- ID情報
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- DOI : 10.1016/j.snb.2015.06.156
- ISSN : 0925-4005
- SCOPUS ID : 84939817236
- Web of Science ID : WOS:000362918100180