2015年1月
BILL-Cadherin/Cadherin-17 Contributes to the Survival of Memory B Cells
PLOS ONE
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回数 : 54
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- 巻
- 10
- 号
- 1
- 開始ページ
- e0117566
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pone.0117566
- 出版者・発行元
- PUBLIC LIBRARY SCIENCE
Memory B cells (MBCs) and long-lived plasma cells (LLPCs) are responsible for immunological "memory", which can last for many years. The long-term survival niche for LLPCs in the bone marrow is well characterized; however, the corresponding niche for MBCs is unclear. BILL-cadherin/cadherin-17 (CDH17) is the only member of the cadherin superfamily that is expressed on mouse B lymphocytes in a spatiotemporally regulated manner. Here, we show that half of all MBCs regain expression of CDH17 during the later stage of development. The maintenance of high affinity antigen-specific serum antibodies was impaired in CDH17(-/-)mice and the number of antigen-specific MBCs was reduced as compared to wildtype mice (WT). Also, specific responses to secondary antigens were ablated in CDH17(-/-)mice, whereas primary antibody responses were the same as those in WT mice. Cell cycle analysis revealed a decline in the proliferation of CDH17-MBCs as compared to CDH17(+) MBCs. In addition, we identified a subpopulation of splenic stromal cells, MAdCAM-1(+) blood endothelial cells (BEC), which was CDH17(+). Taken together, these results suggest that CDH17 plays a role in the long-term survival of MBCs, presumably via an "MBC niche" comprising, at least in part, BEC in the spleen.
- リンク情報
- ID情報
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- DOI : 10.1371/journal.pone.0117566
- ISSN : 1932-6203
- PubMed ID : 25612318
- Web of Science ID : WOS:000348562900036