論文

査読有り
2016年5月

Advanced functionalization of polyhydroxyalkanoate via the UV-initiated thiol-ene click reaction

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Kenji Tajima
  • ,
  • Kosuke Iwamoto
  • ,
  • Yasuharu Satoh
  • ,
  • Ryosuke Sakai
  • ,
  • Toshifumi Satoh
  • ,
  • Tohru Dairi

100
10
開始ページ
4375
終了ページ
4383
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00253-015-7252-3
出版者・発行元
SPRINGER

Polyhydroxyalkanoates (PHAs) incorporating vinyl-bearing 3-hydroxyalkanoates were prepared in 8.5-12.9 g L-1 yield. The molar ratios (0-16 mol%) of the vinyl-bearing 3-hydroxyalkanoate derivatives were controlled by the continuous feeding of undecylenate at various concentrations. Subsequently, the PHAs were functionalized by UV-initiated thiol-ene click reaction and chemical modification. H-1 NMR spectra suggested that 3-mercaptopropionic acid and 2-aminoethanethiol were successfully introduced into the vinyl-bearing PHA. Subsequently, chemical modification using fluorescein or a fibronectin active fragment (GRGDS) was attempted. The former yielded a PHA derivative capable of emitting fluorescence under UV irradiation, which was useful for determining the miscibility of PHA in a composite film comprising poly-EY-lactic acid (PLLA) and PHA. In the latter case, PHA bearing GRGDS peptides exhibited cell adhesiveness, suggesting that its biocompatibility was improved upon peptide introduction. Taken together, the UV-initiated thiol-ene click reaction was demonstrated to be useful in PHA modification.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1007/s00253-015-7252-3
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000375057700011&DestApp=WOS_CPL

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