2016年5月
Advanced functionalization of polyhydroxyalkanoate via the UV-initiated thiol-ene click reaction
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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- 巻
- 100
- 号
- 10
- 開始ページ
- 4375
- 終了ページ
- 4383
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00253-015-7252-3
- 出版者・発行元
- SPRINGER
Polyhydroxyalkanoates (PHAs) incorporating vinyl-bearing 3-hydroxyalkanoates were prepared in 8.5-12.9 g L-1 yield. The molar ratios (0-16 mol%) of the vinyl-bearing 3-hydroxyalkanoate derivatives were controlled by the continuous feeding of undecylenate at various concentrations. Subsequently, the PHAs were functionalized by UV-initiated thiol-ene click reaction and chemical modification. H-1 NMR spectra suggested that 3-mercaptopropionic acid and 2-aminoethanethiol were successfully introduced into the vinyl-bearing PHA. Subsequently, chemical modification using fluorescein or a fibronectin active fragment (GRGDS) was attempted. The former yielded a PHA derivative capable of emitting fluorescence under UV irradiation, which was useful for determining the miscibility of PHA in a composite film comprising poly-EY-lactic acid (PLLA) and PHA. In the latter case, PHA bearing GRGDS peptides exhibited cell adhesiveness, suggesting that its biocompatibility was improved upon peptide introduction. Taken together, the UV-initiated thiol-ene click reaction was demonstrated to be useful in PHA modification.
- リンク情報
- ID情報
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- DOI : 10.1007/s00253-015-7252-3
- ISSN : 0175-7598
- eISSN : 1432-0614
- Web of Science ID : WOS:000375057700011