論文

査読有り
2018年4月3日

Short-term fasting decreases excitatory synaptic inputs to ventromedial tuberoinfundibular dopaminergic neurons and attenuates their activity in male mice

Neuroscience Letters
  • Takafumi Kubota
  • ,
  • Atsushi Fukushima
  • ,
  • Hiroko Hagiwara
  • ,
  • Yoshinori Kamiya
  • ,
  • Miyako Furuta
  • ,
  • Tomoyuki Miyazaki
  • ,
  • Hitomi Fujioka
  • ,
  • Sei-Etsu Fujiwara
  • ,
  • Toshiya Funabashi
  • ,
  • Tatsuo Akema

671
開始ページ
70
終了ページ
75
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.neulet.2018.02.017
出版者・発行元
Elsevier Ireland Ltd

Tuberoinfundibular dopaminergic (TIDA) neurons in the arcuate nucleus (ARC) of the hypothalamus play a role in inhibiting prolactin (PRL) secretion from the anterior pituitary. PRL is involved in a variety of behaviors, including feeding. Consequently, we hypothesized that fasting might reduce the activity of TIDA neurons, which might alter PRL secretion. However, direct examinations of TIDA neuron activity are difficult. Recently, transgenic mice were generated that expressed green fluorescent protein (GFP) under the control of the rat tyrosine hydroxylase gene. We first determined that GFP in the dorsomedial ARC was a reliable marker of TIDA neurons. Then, we performed electrophysiology and immunocytochemistry in GFP-labeled TIDA neurons to examine whether different feeding conditions could change their activity. Eight-week-old male mice were fed or fasted for 24 h. After sacrifice, we prepared acutely isolated brain slices for conducting whole-cell voltage-clamp recordings. TIDA neurons were identified with fluorescence microscopy. The mean amplitude of miniature excitatory postsynaptic currents (mEPSCs) was significantly reduced in fasting mice compared to fed mice, but different feeding conditions did not affect the mean mEPSC intervals. This result suggested that fasting reduced the number of excitatory synaptic inputs to TIDA neurons. To determine whether a reduction in excitatory synaptic inputs would cause a reduction in TIDA neuron activity, we examined the effect of 24-h fasting on c-Fos expression in the ARC. We found that fasting significantly reduced the number of Fos-positive TIDA neurons. In addition, serum PRL levels were significantly increased. Taken together, the present findings suggested that short-term fasting attenuated TIDA neuron activity.

リンク情報
DOI
https://doi.org/10.1016/j.neulet.2018.02.017
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29438798
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85042196107&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85042196107&origin=inward
ID情報
  • DOI : 10.1016/j.neulet.2018.02.017
  • ISSN : 1872-7972
  • ISSN : 0304-3940
  • eISSN : 1872-7972
  • PubMed ID : 29438798
  • SCOPUS ID : 85042196107

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