2008年2月
Galactinol synthase gene of Coptis japonica is involved in berberine tolerance
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 72
- 号
- 2
- 開始ページ
- 398
- 終了ページ
- 405
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.70495
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Many plant secondary metabolites show strong biological activities and are potentially also toxic to plants, while plants producing such active compounds are usually insensitive to their own metabolites, suggesting that they have species-specific detoxification mechanisms. In order to clarify the detoxification mechanism of alkaloids, we used cultured cells of Coptis japonica, which are capable of producing a yellow benzylisoquinoline alkaloid, berberine, and accumulate it in the vacuole. Unlike other plant cells that do not produce berberine, C. japonica shows strong tolerance to this alkaloid. We established a fission yeast strain that was sensitive to berberine and performed functional screening using a C japonica cDNA library. One cDNA clone, which conferred clear berberine tolerance, encoded galactinol synthase (CjGolS). The possible role of CjGolS in berberine tolerance is discussed.
- リンク情報
-
- DOI
- https://doi.org/10.1271/bbb.70495
- CiNii Articles
- http://ci.nii.ac.jp/naid/10027524173
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/18256493
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000253892800017&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1271/bbb.70495
- ISSN : 0916-8451
- eISSN : 1347-6947
- CiNii Articles ID : 10027524173
- PubMed ID : 18256493
- Web of Science ID : WOS:000253892800017