Yusuke Oguchi

Profile Information

Affiliation: Faculty of Science and Engineering, RIKEN(The Institute of Physical and Chemical Research)

Degree: Ph.D(Waseda University)

J-GLOBAL ID: 201001037121060442
researchmap Member ID: 6000023686

Research Areas

Papers

NanoSINC-seq dissects the isoform diversity in subcellular compartments of single cells.

Yusuke Oguchi, Yuka Ozaki, Mahmoud N Abdelmoez, Hirofumi Shintaku

Science advances, 7(15), Apr, 2021
Targeted permeabilization of the cell wall and extraction of charged molecules from single cells in intact plant clusters using a focused electric field

Sangamithirai Subramanian Parimalam, Mahmoud Nady Abdelmoez, Arata Tsuchida, Naoyuki Sotta, Mayuki Tanaka, Takashi Kuromori, Toru Fujiwara, Masami Yokota Hirai, Ryuji Yokokawa, Yusuke Oguchi …

The Analyst, 146(5) 1604-1611, 2021
Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution.

Yusuke Oguchi, Hirofumi Shintaku, Sotaro Uemura

Communications biology, 3(1) 788-788, Dec 18, 2020
Distinct Kinetics in Electrophoretic Extraction of Cytoplasmic RNA from Single Cells.

Mahmoud N Abdelmoez, Yusuke Oguchi, Yuka Ozaki, Ryuji Yokokawa, Hidetoshi Kotera, Hirofumi Shintaku

Analytical chemistry, 92(1) 1485-1492, Jan 7, 2020
Author Correction: A practical guide to intelligent image-activated cell sorting.

Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki …

Nature protocols, 14(11) 3273-3273, Nov, 2019
A practical guide to intelligent image-activated cell sorting.

Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki …

Nature protocols, 14(8) 2370-2415, Aug, 2019
Electrical Lysis and RNA Extraction from Single Cells Fixed by Dithiobis(succinimidyl propionate).

Sangamithirai Subramanian Parimalam, Yusuke Oguchi, Mahmoud N Abdelmoez, Arata Tsuchida, Yuka Ozaki, Ryuji Yokokawa, Hidetoshi Kotera, Hirofumi Shintaku

Analytical chemistry, 90(21) 12512-12518, Nov 6, 2018 Peer-reviewed
Intelligent Image-Activated Cell Sorting.

Nao Nitta, Takeaki Sugimura, Akihiro Isozaki, Hideharu Mikami, Kei Hiraki, Shinya Sakuma, Takanori Iino, Fumihito Arai, Taichiro Endo, Yasuhiro Fujiwaki …

Cell, 175(1) 266-276, Sep 20, 2018
SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology.

Mahmoud N Abdelmoez, Kei Iida, Yusuke Oguchi, Hidekazu Nishikii, Ryuji Yokokawa, Hidetoshi Kotera, Sotaro Uemura, Juan G Santiago, Hirofumi Shintaku

Genome biology, 19(1) 66-66, Jun 6, 2018
Mass spectrometric screening of ligands with lower off-rate from a clicked-based pooled library.

Satoshi Arai, Shota Hirosawa, Yusuke Oguchi, Madoka Suzuki, Atsushi Murata, Shin'ichi Ishiwata, Shinji Takeoka

ACS combinatorial science, 14(8) 451-5, Aug 13, 2012
The role of tropomyosin domains in cooperative activation of the actin-myosin interaction.

Yusuke Oguchi, Junji Ishizuka, Sarah E Hitchcock-DeGregori, Shin'ichi Ishiwata, Masataka Kawai

Journal of molecular biology, 414(5) 667-80, Dec 16, 2011
The bidirectional depolymerizer MCAK generates force by disassembling both microtubule ends.

Yusuke Oguchi, Seiichi Uchimura, Takashi Ohki, Sergey V Mikhailenko, Shin'ichi Ishiwata

Nature cell biology, 13(7) 846-52, May 22, 2011
Insights into the mechanisms of myosin and kinesin molecular motors from the single-molecule unbinding force measurements.

Sergey V Mikhailenko, Yusuke Oguchi, Shin'ichi Ishiwata

Journal of the Royal Society, Interface, 7 Suppl 3 S295-306, Jun 6, 2010
Key residues on microtubule responsible for activation of kinesin ATPase.

Seiichi Uchimura, Yusuke Oguchi, You Hachikubo, Shin'ichi Ishiwata, Etsuko Muto

The EMBO journal, 29(7) 1167-75, Apr 7, 2010
Robust processivity of myosin V under off-axis loads.

Yusuke Oguchi, Sergey V Mikhailenko, Takashi Ohki, Adrian O Olivares, Enrique M De La Cruz, Shin'ichi Ishiwata

Nature chemical biology, 6(4) 300-5, Apr, 2010
Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and function.

Yusuke Oguchi, Sergey V Mikhailenko, Takashi Ohki, Adrian O Olivares, Enrique M De La Cruz, Shin'ichi Ishiwata

Proceedings of the National Academy of Sciences of the United States of America, 105(22) 7714-9, Jun 3, 2008
How the Load and the Nucleotide State Affect the Actin Filament Binding Mode of the Molecular Motor Myosin V.

Sergey V Mikhailenko, Yusuke Oguchi, Takashi Ohki, Togo Shimozawa, Adrian O Olivares, Enrique M De La Cruz, Shin'ichi Ishiwata

The journal of the Korean Physical Society, 53(3) 1726-1730, 2008
2P152 Mapping of kinesin-interaction sites on microtubule(Molecular motors,Poster Presentations)

Uchimura Seiichi, Oguchi Yusuke, Ishiwata Shin'ichi, Muto Etsuko

Seibutsu Butsuri, 47 S151, 2007
Identification of a strong binding site for kinesin on the microtubule using mutant analysis of tubulin.

Seiichi Uchimura, Yusuke Oguchi, Miho Katsuki, Takeo Usui, Hiroyuki Osada, Jun-ichi Nikawa, Shin'ichi Ishiwata, Etsuko Muto

The EMBO journal, 25(24) 5932-41, Dec 13, 2006

Misc.

Key Residues on Microtubules Responsible for Activation of Kinesin ATPase

Seiichi Uchimura, Yusuke Oguchi, You Hachikubo, Shin'ichi Ishiwata, Etsuko Muto

BIOPHYSICAL JOURNAL, 98(3) 370A-370A, Jan, 2010

Research Projects

1細胞内RNA分子とタンパク質分子の同時・多重空間分布解析に向けた基盤技術の開発

科学研究費助成事業, 基盤研究(C), 日本学術振興会, Apr, 2021 - Mar, 2024

小口祐伴
Development of a method of sequence library preparation for the amplification-free single-cell sequencing

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2021

Oguchi Yusuke
Regulatory Basis of Biomotility: Chemo-mechanical Feedback Loop

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (S), Japan Society for the Promotion of Science, Apr, 2010 - Mar, 2016

Ishiwata Shin'ichi, TAKEOKA Shinji, SUZUKI Madoka, MIYAZAKI Makito, ITABASHI Takeshi, Sergey V. Mikhailenko, SHIMOYAMA Isao, FUKUDA Norio, SHIMAMOTO Yuta, OGUCHI Yusuke, OYAMA Kotaro, SHINTANI Seine, TAKAGI Jun, SATO Katsuhiko
染色体分配を制御する微小管脱重合因子の張力発生機構

科学研究費助成事業, 若手研究(B), 日本学術振興会, Apr, 2011 - Mar, 2012

小口祐伴
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