2014年11月
Modern and Simple Construction of Plasmid: Saving Time and Cost
JOURNAL OF MICROBIOLOGY
- ,
- 巻
- 52
- 号
- 11
- 開始ページ
- 891
- 終了ページ
- 897
- 記述言語
- 英語
- 掲載種別
- 書評論文,書評,文献紹介等
- DOI
- 10.1007/s12275-014-4501-6
- 出版者・発行元
- MICROBIOLOGICAL SOCIETY KOREA
Construction of plasmids has been occupying a significant fraction of laboratory work in most fields of experimental biology. Tremendous effort was made to improve the traditional method for constructing plasmids, in which DNA fragments digested with restriction enzymes were ligated. However, the traditional method remained to be a standard protocol more than 40 years. At last, several recent inventions are rapidly and completely replacing the traditional method, because they are far quicker with less cost, and requiring less material. We here introduce three such methods that cover up most of the cases. Moreover, they are complementary with each other. Our lab protocols are provided for "no strain, no pain" construction of plasmids.
- リンク情報
- ID情報
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- DOI : 10.1007/s12275-014-4501-6
- ISSN : 1225-8873
- eISSN : 1976-3794
- Web of Science ID : WOS:000343854600001