論文

査読有り
2015年12月1日

A simple and ultra-low cost homemade seamless ligation cloning extract (SLiCE) as an alternative to a commercially available seamless DNA cloning kit

Biochemistry and Biophysics Reports
  • Yuki Okegawa
  • ,
  • Ken Motohashi

4
開始ページ
148
終了ページ
151
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrep.2015.09.005
出版者・発行元
Elsevier B.V.

The seamless ligation cloning extract (SLiCE) method is a novel seamless DNA cloning tool that utilizes homologous recombination activities in Escherichia coli cell lysates to assemble DNA fragments into a vector. Several laboratory E. coli strains can be used as a source for the SLiCE extract
therefore, the SLiCE-method is highly cost-effective. The SLiCE has sufficient cloning ability to support conventional DNA cloning, and can simultaneously incorporate two unpurified DNA fragments into vector. Recently, many seamless DNA cloning kits have become commercially available
these are generally very convenient, but expensive. In this study, we evaluated the cloning efficiencies between a simple and highly cost-effective SLiCE-method and a commercial kit under various molar ratios of insert DNA fragments to vector DNA. This assessment identified that the SLiCE from a laboratory E. coli strain yielded 30-85% of the colony formation rate of a commercially available seamless DNA cloning kit. The cloning efficiencies of both methods were highly effective, exhibiting over 80% success rate under all conditions examined. These results suggest that SLiCE from a laboratory E. coli strain can efficiently function as an effective alternative to commercially available seamless DNA cloning kits.

リンク情報
DOI
https://doi.org/10.1016/j.bbrep.2015.09.005
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29124198

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