2014年9月
Method for enhancement of plant redox-related protein expression and its application for in vitro reduction of chloroplastic thioredoxins
PROTEIN EXPRESSION AND PURIFICATION
- ,
- 巻
- 101
- 号
- 開始ページ
- 152
- 終了ページ
- 156
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.pep.2014.07.001
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Plant redox-related proteins were overexpressed using a genetic codon substitution downstream of the translation initiation codon. This method significantly improved recombinant protein expression levels of Arabidopsis chloroplastic thioredoxins and cytosolic nicotinamide adenine dinucleotide phosphate (NADPH)-dependent thioredoxin reductase (E.C. 1.8.1.9) in Escherichia coli. Using these proteins, the in vitro chloroplastic thioredoxins-reduction system was reconstituted in an NADPH-dependent manner. This system could convert the five classes of chloroplastic Arabidopsis thioredoxins and two chloroplastic Spinach thioredoxins to their reduced forms, independent of dithiothreitol and the photosynthetic electron transport system. (C) 2014 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.pep.2014.07.001
- ISSN : 1046-5928
- eISSN : 1096-0279
- PubMed ID : 25017253
- Web of Science ID : WOS:000340811000022