論文

2007年9月

Flow cytometric evaluation of synergistic pro-apoptotic effects of statins and clofibrates in im-9 human lymphoblasts

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
  • Naoki Tomiyama
  • ,
  • Noriko Yasuda
  • ,
  • Chihiro Iwano
  • ,
  • Sumio Matzno
  • ,
  • Kenji Matsuyama

34
9
開始ページ
876
終了ページ
880
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/j.1440-1681.2007.04677.x
出版者・発行元
BLACKWELL PUBLISHING

In the present study, we evaluated fibrate-mediated potentiation of statin-induced apoptosis in IM-9 human lymphoblasts.
The pro-apoptotic effects of statin and fibrate were measured by flow cytometry with biotin-annexin V, followed by addition of avidin-fluorescein isothiocyanate and propidium iodide. Apoptosis was confirmed using karyopyknotic staining, as well as detection of DNA fragmentation and caspase 3 activation.
Incubation of IM-9 cells with both 0.1 mu mol/L cerivastatin and 200 mu mol/L clofibrate had a synergistic effect compared with 0.1 mu mol/L cerivastatin alone or 200 mu mol/L clofibrate alone. The magnitude of apoptosis induced by various combinations of statins and clofibrate were as follows: cerivastatin (0.1 mu mol/L) + clofibrate (200 mu mol/L) > atorvastatin (0.1 mu mol/L) + clofibrate (200 mu mol/L) > pravastatin (100 mu mol/L) + clofibrate (200 mu mol/L). Other fibrates (bezafibrate and clinofibrate) did not show any synergistic effect. Furthermore, karyopyknotic staining, caspase 3 activation and DNA fragmentation demonstrated synergistic pro-apoptotic effects of statin and fibrate.
The results of the present study suggest that simultaneous treatment with statins and clofibrate could provide improved therapeutic efficacy in leukaemia patients.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1111/j.1440-1681.2007.04677.x
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000248531700010&DestApp=WOS_CPL

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