論文

査読有り
1998年7月

The identification of DNA binding factors specific for as-1-like sequences in auxin-responsive regions of parA, parB and parC

PLANT AND CELL PHYSIOLOGY
  • T Sakai
  • ,
  • Y Takahashi
  • ,
  • T Nagata

39
7
開始ページ
731
終了ページ
739
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
JAPANESE SOC PLANT PHYSIOLOGISTS

We have identified the auxin-responsive region (Aux-RR) of the parA promoter; it is derived from a gene that is induced by auxin in tobacco mesophyll protoplasts, By analyses of gain-of-function and point mutations in transgenic tobacco plants, we showed that an as-1-like sequence was required, but not alone sufficient, for auxin responsiveness of the parA promoter, as has been also shown for the parC promoter in a recent study. A gel mobility shift assay revealed that the as-1-like sequence of the parA promoter bound specifically to the as-1 binding nuclear factor, ASF-1, while the as-1-like sequence of the parC promoter bound specifically to a novel nuclear factor named ALF-1 (as-1-like sequence binding factor-1). Since these bindings were correlated with auxin-induced activation of transcription, those results suggest that ALF-1 is a new nuclear factor involved in auxin responsiveness and that it is distinct from ASF-1, Furthermore, we found that an as-1-like sequence in the AuxRR of parB bound specifically to a tobacco nuclear factor, named ALF-2, which differed from ASF-1 and ALF-1. These results suggest that the auxin responsiveness of the auxin-inducible genes parA, parB and parC are regulated by different mechanisms, even though the cis-acting elements look similar.

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000074890300007&DestApp=WOS_CPL
URL
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=ISI&KeyUT=WOS:000074890300007&KeyUID=WOS:000074890300007
URL
http://orcid.org/0000-0002-1589-6247
ID情報
  • ISSN : 0032-0781
  • ORCIDのPut Code : 47572042
  • Web of Science ID : WOS:000074890300007

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