2015年8月
Multitarget super-resolution microscopy with high-density labeling by exchangeable probes
NATURE METHODS
- ,
- ,
- ,
- ,
- 巻
- 12
- 号
- 8
- 開始ページ
- 743
- 終了ページ
- U69
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/NMETH.3466
- 出版者・発行元
- NATURE PUBLISHING GROUP
We have developed a multitarget super-resolution microscopy technique called image reconstruction by integrating exchangeable single-molecule localization (IRIS). IRIS uses protein fragment-based probes that directly associate with and dissociate from their targets over durations on the order of tens of milliseconds. By integrating single-molecule localization and sequential labeling, IRIS enables unprecedented labeling density along multiple cellular structures. IRIS can be used to discern the area-specific proximity between cytoskeletal components and focal adhesions within a single cell.
- リンク情報
- ID情報
-
- DOI : 10.1038/NMETH.3466
- ISSN : 1548-7091
- eISSN : 1548-7105
- PubMed ID : 26147917
- Web of Science ID : WOS:000358736100017