香川 憲夫

欧文原著（有審査）

1. Udhane S, Parween S, Kagawa N, Pandey AV (2017) Altered CYP19A1 and CYP3A4 Activities due to Mutations A115V, T142A, Q153R and P284L in the Human P450 Oxidoreductase. Front. Pharmacol. 8, 580.

2. Schiffer L, Anderko S, Hobler A, Hannemann F, Kagawa N, Bernhardt R (2015) A recombinant CYP11B1 dependent Escherichia coli biocatalysist for selective cortisol production and optimization towards a preparative scale. Microbial Cell Factories, 14, 209.

3. Zhao B, Lei L, Kagawa N, Sundaramoorthy M, Banerjee S, Nagy LD, Guengerich FP, Waterman MR. (2012) Three-dimensional structure of steroid 21-hydroxylase (cytochrome P450 21A2) with two substrates reveals locations of disease-associated variants. J Biol Chem, 287, 10613-10622.

4. Hobler A, Kagawa N, Hutter MC, Hartmann MF, Wudy SA, Hannemann F, Bernhardt R. (2012) Human aldosterone synthase: recombinant expression in E. coli and purification enables a detailed biochemical analysis of the protein on the molecular level. J Steroid Biochem Mol Biol, 132, 57-65.

5. Haneishi A, Takagi K, Asano K, Nakamura S, Kagawa N, Yamada K. (2011) Genistein stimulates the insulin-dependent signaling pathway. Front Biosci, E3, 1534-1540.

6. Khatri Y, Hannemann F, Ewen KM, Pistorius D, Perlova O, Kagawa N, Brachmann AO, Müller R, Bernhardt R. (2011) The CYPome of Sorangium cellulosum So ce56 and identification of CYP109D1 as a new fatty acid hydroxylase. Chem Biol, 17, 1295-305.

7. Kaneko K, Kumekawa Y, Makino R, Nozawa H, Hirayama Y, Kogo M, Konishi K, Katagiri A, Kubota Y, Muramoto T, Kushima M, Ohmori T, Oyama T, Kagawa N, Ohtsu A, Imawari M (2010) EGFR gene alterations as a prognostic biomarker in advanced esophageal squamous cell carcinoma. Front Biosci, 15, 65-72.

8. Oyama Y, Nagayoshi H, Matsuda T, Oka M, Isse T, Yu H-S, Pham T-T-P, Tanaka M, Kagawa N, Kaneko K, Kawamoto T (2010) N2-ethylidene-dG DNA adducts in Aldh2 KO mice treated with acetaldehyde inhalation, Front Biosci, E2, 1344-1354.

9. Yashima Y, Oyama T, Pham TT, Yamaguchi T, Narai R, Isse T, Uramoto H, Sugio K, Yasumoto K, Kagawa N, Kawamoto T. (2009) Hazardous environmental factors enhance impairment of liver function in HBV and HCV hepatitis. Front Biosci, 14, 4071-4078.

10. Yamada K, Ogata-Kawata H, Matsuura K, Kagawa N, Takagi K, Asano K, Haneishi A, Miyamoto K. (2009) ZHX2 and ZHX3 repress cancer markers in normal hepatocytes. Front Biosci, 14, 3724-3732.

11. Oyama, T., Kagawa, N., Sugio, K., Uramoto, H., Hatano, O., Harada, N., Kaneko, K., Kawamoto, T., Yasumoto, K. (2009) Expression of aromatase CYP19 and its relationship with parameters in NSCLC. Front Biosci, 14, 2285-2292.

12. Miller, TW., Shin, I., Kagawa, N., Evans, DB., Waterman, MR., and Arteaga, CL. (2008) Aromatase is phosphorylated in situ at Serine-118. J Steroid Biochem Mol Biol., 112, 95-101.

13. Grinkova, Y., Kagawa, N., Waterman, MR., and Sligar, S. (2008) The Ferrous-Oxygen Complex of Human Aromatase (CYP19). Biochem Biophys Res Commun, 372, 379-82.

14. Zollner, A., Kagawa, N., Waterman, MR., Nonaka, Y., Takio, K., Shiro, Y., Hannemann, F., and Bernhardt, R. (2008) Purification and functional characterization of human 11β-hydroxylase (hCYP11B1) expressed in E. coli. FEBS J., 275, 799-810.

15. Tosha, T., Kagawa, N., Arase, M., Waterman, M. R., and Kitagawa, T. (2008) Interaction between substrate and oxygen ligand responsible for effective O-O bond cleavage in bovine cytochrome P450 steroid 21-hydroxylase proved by Raman spectroscopy. J. Biol. Chem. 283, 3708-3717.

16. Oyama, T., Sugio, K., Uramoto, H., Iwata, T., Onitsuka, T., Isse, T., Nozoe, T., Kagawa, N., Yasumoto, K., and Kawamoto, T. (2007) Increased cytochrome P450 and aryl hydrocarbon receptor in bronchial epithelium of heavy smokers with non-small cell lung carcinoma carries a poor prognosis. Front. Biosci., 12, 4497-4503.

17. Oyama, T., Sugio, K., Uramoto, H., Kawamoto, T., Kagawa, N., Nadaf, S., Carbone, D. P., and Yasumoto, K. (2007) Cytochrome P450 expression (CYP) in non-small cell lung cancer. Front. Biosci., 12, 2299-308.

18. Ogata-Kawata, H., Yamada, K., Uesaka-Yoshino, M., Kagawa, N., and Miyamoto, K. (2007) BS69, a corepressor interacting with ZHX1, is a bifunctional transcription factor. Front. Biosci., 12:1911-26.

19. Rupasinghe, S., Schuler, M. A., Kagawa, N., Yuan, H., Lei, L., Zao, B., Kelly, S. L., Waterman, M. R., and Lamb, D. C. (2006) The cytochrome P450 gene family CYP157 does not contain EXXR in the K-helix reducing the absolute conserved P450 residues to a single cysteine. FEBS Lett., 580, 6338-42.

20. Arase, M., Waterman, M.R., and Kagawa, N. (2006) Purification and characterization of steroid 21-hydroxylase P450c21 efficiently expressed in E. coli. Biochem Biophys Res Commun, 344, 400-405.

21. Tosha, T., Kagawa, N., Ohta, T., Yoshioka, S., Waterman, M.R., and Kitagawa, T. (2006) Raman Evidence for Specific Substrate-induced Structural Changes in the Heme Pocket of Human Cytochrome P450 Aromatase during the Three Consecutive Oxygen Activation Steps. Biochemistry, 45, 5631-5640.

22. Urushino, N., Yamamoto, K., Kagawa, N., Ikushiro, S., Kamakura, M., Yamada, S., Kato, S., Inouye, K., and Sakaki, T. (2006) Interaction between mitochondrial CYP27B1 and adrenodoxin: Role of Arg458 of Mouse CYP27B1, Biochemistry, 45, 4405-4412.

23. Ozaki, S., Oyama, T., Toyohi, I., Kagawa, N., Uramoto, H., Sugio, K., Yasumoto, K., Kono, K., and Kawamoto, T. (2006) Smoking cessation program and CYP2A6 polymorphism. Front Biosci, 11, 2590-2597.

24. Yamamoto, K., Uchida, E., Urushino, N., Sakaki, T., Kagawa, N., Sawada, N., Kamakura, M., Kato, S., Inouye, K., Yamada, S. (2005) Identification of Amino Acid Residue of CYP27B1 Responsible for Binding of 25-Hydroxyvitamin D3 Whose Mutation Causes Vitamin D-Dependent Rickets Type I. J Biol Chem, 280, 30511-30516.

25. Morita, M., Oyama, T., Kagawa, N., Nakata, S., Ono, K., Sugaya, M., Uramoto, H., Yoshimatsu, T., Hanagiri, T., Sugio, K., Kakeji, Y., Yasumoto, K. (2005) Expression of aldehyde dehydrogenase 2 in the normal esophageal epithelium and alcohol consumption in patients with esophageal cancer. Front Biosci, 10, 2319-2324.

26. Matsuura, K., Yoshioka, S., Tosha, T., Uchida, T., Hori, H., Takahashi, S., Ishimori, K., Kitagawa, T., Morishima, I., Kagawa, N., and Waterman, M.R. (2005) Structural Diversities in Clinical Azole Bound Forms Between Sterol 14α-Demethylases From Human And Mycobacterium Tuberculosis. J Biol Chem, 280, 9088-96.

27. Oyama, T., Morita, M., Isse, T., Kagawa, N., Nagata, Y., So, T., Mizukami, M., Ichiki, Y., Ono, K., Sugaya, M., Uramoto, H., Yoshimatsu, T., Hanagiri, T., Sugio, K., Kawamoto, T., and Yasumoto, K. (2005) Immunohistochemical Evaluation of Cytochrome P450 (CYP) And p53 in Breast Cancer. Front Biosci, 10, 1156-1161.

28. Oyama, T., Isse, T., Kagawa, N., Kinaga, T., Kim, Y-D., Morita, M., Sugio, K., Weiner, H., Yasumoto, K., and Kawamoto, T. (2005) Tissue-Distribution of Aldehyde Dehydrogenase 2 And Effects of the Aldh2 Gene-Disruption on the Expression of Enzymes Involved in Alcohol Metabolism, Front Biosci, 10, 951-60.

29. Kagawa, N., Senbonmatsu, T., Satoh, K., Ichihara, K., Yamagata, N., Hatano, O., Saito, T., Nguyen, V. Q., Waterman, M. R., Price, E. J., Atkinson, J. B., and Inagami. T., (2005) Tetracycline Protects Myocardium Against Ischemic Injury. Front Biosci, 10, 608-619.

30. Uchida, E., Kagawa, N., Sakaki, T., Urushino, N., Sawada, N., Kamakura, M., Ohta, M., Kato, S., and Inoue, K. (2004) Purification And Characterization of Mouse CYP27B1 Overproduced by an Escherichia coli System Coexpressing Molecular Chaperonins GroEL/ES. Biochem Biophys Res Commun, 323, 505-11.

31. Kagawa, N., Hara, H., Waterman, MR, and Yoshioka, S. (2004) Characterization of Stable Human Aromatase (P450arom) Expressed in E. coli. Steroids, 69, 235-243.

32. Kagawa, N., Cao. Q., and Kusano, K. (2003) Expression of Human Aromatase (CYP19) in Escherichia coli by N-Terminal Replacements And Induction of Cold Stress Response. Steroids, 68, 205-209.

33. Sewer, M. B., Nguyen, V., Huang, C-J, Tucker, P. W., Kagawa, N., and Waterman, M. R. (2002) Transcriptional Activation of Human CYP17 in H297R Adrenocortical Cells Depends on Complex Formation Between p54nrb/NonO, PSF And SF-1, a Complex Which Also Participates in Repression of Transcription. Endocrinology, 143, 1280-1290.

34. Kagawa, N. and Cao, Q. (2001) Osmotic Stress Induced by Carbohydrates Enhances Expression of Foreign Proteins in Escherichia coli. Arch Biochem Biophys, 393, 290-296. 3

35. Kusano, K., Kagawa, N., Sakaguchi, M., Omura, T., and Waterman, M. R. (2001) Importance of a Proline-Rich Sequence in the Amino-Terminal Region for Correct Folding of Mitochondrial And Soluble Microbial P450s. J Biochem (Tokyo), 129, 271-277.

36. Kusano, K., Sakaguchi, M., Kagawa, N., Waterman, M.R., and Omura, T. (2001) Microsomal P450s Use Specific Proline-Rich Sequences for Efficient Folding But Not Maintainance of the Folded Structure. J Biochem (Tokyo), 129, 259-269.

37. Cheng, P-Y., Kagawa, N., Takahashi, A., and Waterman, M.R. (2000) Three Zinc Finger Nuclear Proteins, Sp1, Sp3, and a ZBP-89 Homolog, Bind to the cAMP-Responsive Sequence of the Bovine Adrenodoxin Gene and Regulate Transcription. Biochemistry, 39, 4347-4357.

38. Kusano, K., Waterman, M.R., Sakaguchi, M., Omura, T., and Kagawa, N. (1999) Protein Synthesis Inhibitors And Ethanol Selectively Enhance the Heterologous Expression of P450 And Related Proteins in Escherichia coli. Arch Biochem Biophys, 367, 129-136.

39. Bischof, L.J., Kagawa, N., and Waterman, M.R. (1998) The Bovine CYP17 Promoter Contains a Transcriptional Regulatory Element Cooperatively Bound by TALE Homeodomain Proteins. Endocrin Research, 24, 489-495.

40. Nonaka, Y., Fujii, T., Kagawa, N., Waterman, M.R., Takemori, H., and Okamoto, M. (1998) Structure/Function Relation of CYP11B1 Associated with Dahl's Salt-Resistant Rats. Expression of rat CYP11B1 and CYP11B2 in Escherichia coli. Eur J Biochem, 258, 869-878.

41. Bishof, L., Kagawa, N., Moskow, J.J., Takahashi, Y., Iwamatsu, A., Steelman, S., Buchberg, A.M., and Waterman, M.R.(1998) Members of the Meis1 and Pbx Homeodomain Protein Families Cooperatively Bind a cAMP Responsive Sequence (CRS1) from Bovine CYP17. J Biol Chem, 273, 7941-7948.

42. Katagiri, M., Tatsuta, K., Imaoka, S., Funae, Y., Honma, K., Matsuo, N., Yokoi, H., Ishimura, k., Ishibashi, F., Kagawa, N. (1998) Evidence that Immature Rat Liver Is Capable of Participating in Steroidogenesis by Expressing 17α-Hydroxylase/17,20-Lyase P450c17. J Steroid Biochem Mol Biol, 64, 121-128.

43. Ogo, A., Waterman, M.R., McAllister, J.M., and Kagawa, N. (1997) The homeodomain protein Pbx1 is involved in cAMP-dependent transcription of human CYP17. Arch Biochem Biophys, 348, 226-231.

44. Jenkins C.M., Pikuleva, I., Kagawa, N., Waterman, M.R. (1997) Escherichia coli Flavodoxin Sepharose as an Affinity Resin for Cytochromes P450 and Use to Identify a Putative Cytochrome P450c17/3β-Hydroxysteroid Dehydrogenase Interaction. Arch Biochem Biophys, 347, 93-102.

45. Ogo, A., and Waterman, M.R., Kagawa, N. (1997) cAMP-Dependent Transactivation Involving the Homeodomain Protein Pbx1. Arch Biochem Biophys, 338, 193-200.

46. Ogo, A., Waterman, M.R., Kamps, M.P., Kagawa, N. (1995) Protein Kinase A-Dependent Transactivation by the E2A-Pbx1 Fusion Protein. J Biol Chem, 270, 25340-25343.

47. Katagiri, M., Kagawa, N., and Waterman, M.R. (1995) The Role of Cytochrome b5 in the Biosynthesis of Androgens by Human P450c17. Arch Biochem Biophys, 317 343-347.

48. Pikureva, I.A., Mackman, R.L., Kagawa, N., Waterman, M.R., and Ortiz de Montellano, P.R. (1995) Active Site Topology of Bovine Cholesterol Side-chain Cleavage Cytochrome P450 (P450scc) And Evidence for Interaction of Tyrosine 94 with the Side-chain of Cholesterol. Arch Biochem Biophys, 322, 189-197.

49. Kagawa, N., Ogo, A., Takahashi, Y., Iwamatsu, A., and Waterman, M.R. (1994) A cAMP-Responsive Sequence (CRS1) of CYP17 Is a Cellular Target for the Homeodomain Protein Pbx1. J Biol Chem, 269 18716-18719.

50. Imai, T., Globerman, H., Gertner, J.M., Kagawa, N., and Waterman, M.R. (1993) Expression and Purification of Functional Human 17α-Hydroxylase/17,20-lyase(P450c17) in Escherichia coli. J Biol Chem, 268 19681-19689.

51. Lauber,M.E., Kagawa, N., Waterman, M.R., and Simpson, E.R. (1993) cAMP-dependent and Tissue-specific Expression of Genes Encoding Steroidogenic Enzymes in Bovine Luteal and Granulosa Cell in Primary Culture. Mol Cell Endocrinol, 93 227-233.

52. Kagawa, N. and Waterman, M.R. (1992) Purification And Characterization of a Transcription Factor Which Appears to Regulates the cAMP-responsiveness of the Human CYP21B Gene. J Biol Chem, 267 25213-25219.

53. Kagawa, N. and Waterman, M.R. (1991) Evidence that an Adrenal-Specific Nuclear Protein Regulates the cAMP Responsiveness of the Human CYP21B (P450C21) Gene. J Biol Chem, 266, 11199-11204.

54. Mathew, P., Kagawa, N., Bhasker, C.R. and Waterman, M.R. (1990) Deduced Amino Acid Sequence of Heme Binding Region of Chicken Cholesterol Side Chain Cleavage Cytochrome P450. Protein Sequence and Data Analysis, 3, 323-325.

55. Kagawa, N. and Waterman, M.R. (1990) cAMP-Dependent Transcription of the Human CYP21B (P450C21) Gene Requires a Cis-Regulatory Element Distinct from the Consensus cAMP-Regulatory Element. J Biol Chem, 256, 11299-11305.

56. Kagawa, N., Mihara, K. and Sato, R. (1987) Structural Analysis of Cloned cDNA for Polycyclic Hydrocarbon-Inducible Forms of Rabbit Liver Microsomal Cytochrome P450. J Biochemistry, 101, 1471-1479.

57. Hata, T., Sekine, M. and Kagawa, N. (1975) Silyl Phosphite II. Reactions of Tris-(trimethylsilyl) Phosphite with Alkyl Halides. Chemistry Letters, 7, 635-636.

欧文総説
1. Oyama T, Uramoto H, Kagawa N, Yoshimatsu T, Osaki T, Nakanishi R, Nagaya H, Kaneko K, Muto M, Kawamoto T, Tanaka F, Gotoh A (2012) Cytochrome P450 in non-small cell lung cancer related to exogenous chemical metabolism. Front Biosci (Schol Ed), 4, 1539-1546.

2. Oyama, T., Sugio, K., Isse, T., Matsumoto, A., Uramoto, H., Nozoe, T., Morita, M., Kagawa, N., Muto, M., Yasumoto, K., and Kawamoto, T. (2008) Expression of Cytochrome P450 in non-small cell lung cancer. Front Biosci, 13, 5787-5793.

3. Sakaki, T., Kagawa, N., Yamamoto, K., and Inouye, K. (2005) Metabolism of Vitamin D3 by Cytochromes P450. Front Biosci, 10, 119-134.

4． Oyama, T., Kagawa, N., Kunugita, N., Kitagawa, K., Masanori Ogawa, M., Tetsunosuke Yamaguchi, T., Suzuki, R., Kinaga, T., Yashima, Y., Ozaki, S., Isse, T., Kim, Y., Kim, H., and Kawamoto, T. (2004) Expression of Cytochrome P450 in Tumor Tissues And Its Association with Cancer Development. Front Biosci, 9, 1967-1976.

5. Oyama, T., Kagawa, N., Matsumoto, A., Isse, T., and Kawamoto, T. (2003) Lung Cancer And CYP1A1 or GSTM1 Polymorphisms. Environmental Health and Preventive Medicine, 7, 230-234.

6. Kagawa, N., Bischof, L.J., Cheng, P-Y., Anwar, A., and Waterman, M.R. (1999) Biochemical Diversity of Peptide-Hormone Dependent Regulation of Steroidogenic P450s. Drug Metabolism Reviews, 31, 333-342.

7. Waterman, M.R., Kagawa, N., Zanger, U.M., Momoi, K., Lund, J., and Simpson, E.R. (1992) Comparison of cAMP-Responsive DNA Sequences and Their Binding Proteins Associated with Expression of the Bovine CYP17 and CYP11B and Human CYP21B Genes. J Steroid Biochem Molec Biol, 43, 931-935.

8. Zanger, U.M., Kagawa, N., Lund, J. and Waterman, M.R. (1992) Distinct Biochemical Mechanisms for cAMP-Dependent Transcription of CYP17 and CYP21. FASEB J, 6, 719-723.


欧文著書
58. T1. Kagawa, N. (2011) Chapter Title “Efficient Expression of Human Aromatase (CYP19) in E. coli” in “Heterologous Gene Expression In E. Coli” edited by Thomas C. Evans, Jr. and Ming-Qun Xu in the series, Methods in Molecular Biology, Humana Press Inc. Vol 705, 109-122.

T2. Kagawa, N. and Cao, Q. (2001) Stress response and foreign gene expression in E. coli. In "Recent Research Developments in Biophysics and Biochemistry", Research Signpost, vol 1, 99-107.

T3. Kagawa, N. and Waterman, M.R. (1995) Chapter 12, Regulation of Synthesis and Activity of Steroidogenic Cytochrome P450s in 'Cytochrome P450: Structure, Mechanism, and Biochemistry'. Ortiz de Montellano, P. Ed., Plenum, New York. 419-442.


和文総説
JR1. 香川憲夫、片桐昌直（2003）「大腸菌内での外来性タンパク質の発現 ：P450の発現を通して。」生化学、75, 279-284.

JR2. 香川憲夫、草野数富、野中泰樹 (2000) 「大腸菌におけるホ乳類 Cytochrome P450 活性酵素タンパク質の発現。」生物工学会誌、78, 82-93.

JR3. 香川憲夫(2000) 「阻害剤は促進する？」生物工学会誌、78, 63.

JR4. 片桐昌直、香川憲夫 (1998) 「ヒト17α-hydroxylase/17,20-lyase (P450c17) によるステロイドホルモン代謝調節。」 日薬理誌、 112, 43-50.