論文

査読有り
2009年12月

The bHLH transcription factor Hand2 regulates the expression of nanog in ANS differentiation

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Yusuke Hashimoto
  • ,
  • Reiko Myojin
  • ,
  • Naohiro Katoh
  • ,
  • Masaya Ohtsu
  • ,
  • Fumio Tashiro
  • ,
  • Fumitoshi Onoda
  • ,
  • Yasufumi Murakami

390
2
開始ページ
223
終了ページ
229
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2009.09.090
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

The basic helix-loop-helix transcription factor Hand2 is induced by bone morphogenetic proteins (BMPs) in neural crest-derived precursor cells during the early stage of development of the autonomic nervous system (ANS). Previous studies showed that Hand2 was essential for the ANS differentiation. However, regulatory mechanism of pluripotent genes has not been elucidated in ANS differentiation. Here, we show that Hand2 regulated nanog expression in ANS differentiation. Our studies demonstrated that the forced expression of Hand2 promoted the ANS differentiation program in P19 embryonal carcinoma (EC) cells without aggregation. Furthermore, Our results suggested that Hand2 bound to the promoter of nanog, a gene required for embryonic stem cells self-renewal, and suppressed nanog expression after Hand2 induction. The rapid downregulation of nanog mRNA during ANS differentiation correlated with the Hand2 transcriptional activity and nanog promoter methylation. These findings are evidence for a presence of the novel regulatory mechanism of nanog in ANS differentiation. (C) 2009 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2009.09.090
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19785992
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000271552400010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbrc.2009.09.090
  • ISSN : 0006-291X
  • PubMed ID : 19785992
  • Web of Science ID : WOS:000271552400010

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