論文

査読有り
2009年9月

Development of effective isolation method of ES cells for analysis of differentiation

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Kensuke Ohse
  • ,
  • Masaya Ohtsu
  • ,
  • Fumitoshi Onoda
  • ,
  • Yasufumi Murakami

387
1
開始ページ
64
終了ページ
69
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2009.06.122
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Neuroectoderm development is a milestone of vertebrate neurogenesis. However, the molecular mechanism underlying the differentiation of neuroectoderm is still unclear, especially in mammals. ES cells co-cultured with PA6 cells can differentiate to neuroectoderm by the stromal cell-derived inducing activity method (SDIA method), but contamination of PA6 cells is an obstacle to the analysis of molecular mechanisms of differentiation. Here we describe a novel method by which differentiated ES cells are easily isolated from PA6 cells. We attempted to induce the differentiation of ES cells using paraformaldehyde-fixed PA6 cells. RT-PCR and DNA microarray analysis revealed that the background noise derived from contaminated PA6 cells disappeared when fixed PA6 cells were used. Furthermore, genes up-regulated during the differentiation of ES cells were expressed in a developing mouse embryo. Thus, our newly developed method will be very useful for identifying novel genes associated with mouse neuroectoderm development in vitro and in vivo. (C) 2009 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2009.06.122
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19559667
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000268736100012&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbrc.2009.06.122
  • ISSN : 0006-291X
  • PubMed ID : 19559667
  • Web of Science ID : WOS:000268736100012

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