論文

査読有り 国際誌
2008年8月

Novel DNA microarray system for analysis of nascent mRNAs.

DNA research : an international journal for rapid publication of reports on genes and genomes
  • Masaya Ohtsu
  • ,
  • Mika Kawate
  • ,
  • Masashi Fukuoka
  • ,
  • Wataru Gunji
  • ,
  • Fumio Hanaoka
  • ,
  • Takahiko Utsugi
  • ,
  • Fumitoshi Onoda
  • ,
  • Yasufumi Murakami

15
4
開始ページ
241
終了ページ
51
記述言語
英語
掲載種別
DOI
10.1093/dnares/dsn015
出版者・発行元
4

Transcriptional activation and repression are a key step in the regulation of all cellular activities. The development of comprehensive analysis methods such as DNA microarray has advanced our understanding of the correlation between the regulation of transcription and that of cellular mechanisms. However, DNA microarray analysis based on steady-state mRNA (total mRNA) does not always correspond to transcriptional activation or repression. To comprehend these transcriptional regulations, the detection of nascent RNAs is more informative. Although the nuclear run-on assay can detect nascent RNAs, it has not been fully applied to DNA microarray analysis. In this study, we have developed a highly efficient method for isolating bromouridine-labeled nascent RNAs that can be successfully applied to DNA microarray analysis. This method can linearly amplify small amounts of mRNAs with little bias. Furthermore, we have applied this method to DNA microarray analysis from mouse G2-arrested cells and have identified several genes that exhibit novel expression profiles. This method will provide important information in the field of transcriptome analysis of various cellular processes.

リンク情報
DOI
https://doi.org/10.1093/dnares/dsn015
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18611946
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2575885
ID情報
  • DOI : 10.1093/dnares/dsn015
  • ISSN : 1340-2838
  • PubMed ID : 18611946
  • PubMed Central 記事ID : PMC2575885

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