2011年9月
An enzymatic colorimetric quantification of orthophosphate
Journal of Applied Glycoscience
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- 巻
- 58
- 号
- 3
- 開始ページ
- 125
- 終了ページ
- 127
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.5458/jag.jag.JAG-2011_002
- 出版者・発行元
- The Japanese Society of Applied Glycoscience
Quantification of orthophosphate (Pi) in the presence of labile phosphate esters is required for biochemical assays. We developed a method for the enzymatic colorimetric quantification of Pi using pyruvate oxidase and peroxidase. The calibration curve was not affected by the presence of labile phosphate esters. Furthermore, this method allows continuous monitoring of the reaction of Pi-releasing enzymes.
- リンク情報
- ID情報
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- DOI : 10.5458/jag.jag.JAG-2011_002
- ISSN : 1344-7882
- CiNii Articles ID : 10029656849
- CiNii Books ID : AA11809133