Serpentine is considered the main hydrous mineral to cause significant weakening at the slab-mantle interface; however, despite the possible presence of lizardite/cluysotile (liz/ctl) and antigorite (i.e., low- and high-temperature serpentine species, respectively) at the base of the mantle wedge, the differences in rheological properties between these two serpentine types are poorly understood. To investigate the effect of serpentine speciation on slab-mantle decoupling, we performed a series of two-layer shear deformation experiments on liz/ctl, antigorite, and olivine at P = 1 GPa and T = 250-300 degrees C, using a Griggs-type solid-medium apparatus. With increasing shear strain (gamma up to similar to 5), the liz/ctl and antigorite samples evolved to deform predominantly by slip or glide on the (001) basal plane, whereas the olivine sample deformed by a combination of brittle and crystal-plastic mechanisms. The contrasting amount of rotation of strain markers between layers demonstrates that the shear strain of liz/ctl is significantly lower than that of antigorite, possibly arising from differences in interlayer bond strength. The strain contrasts between serpentines and olivine were gamma(liz/ctl)/gamma(ol) = 9-12 and gamma(atg)/gamma(ol) = 1-2. Our results indicate that assuming a thin, serpentinized layer upon a subducting slab, significant decoupling can occur only where liz/ctl is stable in the layer. Thus, the distribution of serpentine species in the hydrated mantle wedge is a critical factor that controls the degree of decoupling along the slab-wedge interface, at depths below the seismogenic zone. (C) 2013 Elsevier B.V. All rights reserved.