2012年6月
KRAS up-regulates the expression of miR-181a, miR-200c and miR-210 in a three-dimensional-specific manner in DLD-1 colorectal cancer cells
Anticancer Research
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- 巻
- 32
- 号
- 6
- 開始ページ
- 2271
- 終了ページ
- 2276
- 記述言語
- 英語
- 掲載種別
Background: We previously found that oncogenic KRAS induces increased expression of microRNAs (miRNAs), such as miR-200c and miR-221/222, in human colorectal cancer (CRC) HCT116 cells in a three-dimensional (3D)-specific manner, however, the regulation of miRNA expression through oncogenic KRAS in other types of CRC remains unclear. Materials and Methods: The differential expression of 94 cancer-related miRNAs was examined in DLD-1 and DKO-4 cells (DLD-1 cells with a disrupted oncogenic KRAS) in 3D cultures. Results: Increased miR-15b, miR-16, miR- 23a, miR-24, miR-103 and miR-222 expression was observed in 3D and in 2D cultures. Of note, increased miR-181a, miR- 200c and miR-210 expression was only observed in 3D cultures. Furthermore, miR-181a and miR-210 were significantly overexpressed in DLD-1 cells in 3D culture compared with those in HCT116 cells, and were significantly overexpressed in human CRC specimens. Conclusion: Oncogenic KRAS regulates 3D-specific miRNAs that are possibly associated with CRC development in vivo.
- リンク情報
- ID情報
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- ISSN : 0250-7005
- PubMed ID : 22641662
- SCOPUS ID : 84864569241