2014年4月24日
Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation.
Scientific reports
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- 巻
- 4
- 号
- 開始ページ
- 4781
- 終了ページ
- 4781
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep04781
Electrical impulses are necessary for proper in vivo skeletal muscle development. To fabricate functional skeletal muscle tissues in vitro, recapitulation of the in vivo niche, including physical stimuli, is crucial. Here, we report a technique to engineer skeletal muscle tissues in vitro by electrical pulse stimulation (EPS). Electrically excitable tissue-engineered skeletal muscle constructs were stimulated with continuous electrical pulses of 0.3 V/mm amplitude, 4 ms width, and 1 Hz frequency, resulting in a 4.5-fold increase in force at day 14. In myogenic differentiation culture, the percentage of peak twitch force (%Pt) was determined as the load on the tissue constructs during the artificial exercise induced by continuous EPS. We optimized the stimulation protocol, wherein the tissues were first subjected to 24.5%Pt, which was increased to 50-60%Pt as the tissues developed. This technique may be a useful approach to fabricate tissue-engineered functional skeletal muscle constructs.
- リンク情報
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- DOI
- https://doi.org/10.1038/srep04781
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/24759171
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998029
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899474671&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84899474671&origin=inward
- ID情報
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- DOI : 10.1038/srep04781
- eISSN : 2045-2322
- PubMed ID : 24759171
- PubMed Central 記事ID : PMC3998029
- SCOPUS ID : 84899474671