論文

査読有り 国際誌
2014年4月24日

Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation.

Scientific reports
  • Akira Ito
  • ,
  • Yasunori Yamamoto
  • ,
  • Masanori Sato
  • ,
  • Kazushi Ikeda
  • ,
  • Masahiro Yamamoto
  • ,
  • Hideaki Fujita
  • ,
  • Eiji Nagamori
  • ,
  • Yoshinori Kawabe
  • ,
  • Masamichi Kamihira

4
開始ページ
4781
終了ページ
4781
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep04781

Electrical impulses are necessary for proper in vivo skeletal muscle development. To fabricate functional skeletal muscle tissues in vitro, recapitulation of the in vivo niche, including physical stimuli, is crucial. Here, we report a technique to engineer skeletal muscle tissues in vitro by electrical pulse stimulation (EPS). Electrically excitable tissue-engineered skeletal muscle constructs were stimulated with continuous electrical pulses of 0.3 V/mm amplitude, 4 ms width, and 1 Hz frequency, resulting in a 4.5-fold increase in force at day 14. In myogenic differentiation culture, the percentage of peak twitch force (%Pt) was determined as the load on the tissue constructs during the artificial exercise induced by continuous EPS. We optimized the stimulation protocol, wherein the tissues were first subjected to 24.5%Pt, which was increased to 50-60%Pt as the tissues developed. This technique may be a useful approach to fabricate tissue-engineered functional skeletal muscle constructs.

リンク情報
DOI
https://doi.org/10.1038/srep04781
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24759171
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998029
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899474671&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84899474671&origin=inward
ID情報
  • DOI : 10.1038/srep04781
  • eISSN : 2045-2322
  • PubMed ID : 24759171
  • PubMed Central 記事ID : PMC3998029
  • SCOPUS ID : 84899474671

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